Molecular integration in neuron in vivo

体内神经元的分子整合

• 批准号: 7910728
• 负责人: AKIRA CHIBA
• 金额: $ 33.75万
• 依托单位: UNIVERSITY OF MIAMI CORAL GABLES
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7910728
• 关键词: Accounting; Bioprobe; Cell Cycle; Cells; Development; Drosophila genus; Energy Transfer; Goals; Grant; Guanosine Triphosphate Phosphohydrolases; In Situ; Individual; Lead; Molecular; Neurons; Property; Resolution; Role; Signal Transduction; Time; base; design; in vivo; nanoscale; receptor; spatiotemporal

DESCRIPTION (provided by applicant): This project develops molecular Bioprobes that can be introduced into Drosophila neurons. These Bioprobes are designed to reveal dynamic signal integrations within an individual neuron in situ. During the initial grant period, the project develops new FRET (fluorescent resonance energy transfer)-based nano-scale Bioprobes to study Cdc42 signaling at the onset of dendrogenesis. The molecular mechanisms of dendrogenesis are not well understood. The complexity among cells and molecules in the CNS requires an approach that supports high-resolution analysis. Recent advances in genetically encoded Bioprobes allow an unprecedented opportunity to launch this new study. Preliminary observations lead to the hypothesis that spatiotemporal integration of Cdc42 activation and effector availability accounts for the precise space-time at which dendrogenesis initiates in neuron in vivo. Therefore, the goal for the initial grant period is two-fold: (1) to examine the role of Cdc42 signaling at the onset of dendrogenesis, and (2) to demonstrates the power of a Bioprobe-assisted in vivo approach. The Bioprobe-assisted analysis is readily applicable to studies on various properties of a neuron and its compartments.

描述（由申请人提供）：该项目开发了可以引入果蝇神经元的分子生物探测器。 这些生物探测器旨在揭示单个神经元内的动态信号积分。 在最初的赠款期间，该项目开发了新的FRET（荧光共振能量转移）基于基于纳米级的生物探测器，以在树突生成开始时研究CDC42信号。 树突生成的分子机制尚不清楚。 中枢神经系统中细胞和分子之间的复杂性需要一种支持高分辨率分析的方法。 遗传编码的生物探测器的最新进展为启动这项新研究提供了前所未有的机会。 初步观察结果导致了以下假设：Cdc42激活和效应子的时空整合是在体内神经元开始的确切时空的确切时空。 因此，初始赠款期的目标是两个方面：（1）检查Cdc42信号在树突生成开始时的作用，（2）证明在体内方法中生物探针辅助的功能。 生物探针辅助分析很容易适用于神经元及其隔室的各种特性的研究。