ROLE OF HOMEODOMAIN GENES IN HUMAN PROSTATE CANCER

同源域基因在人类前列腺癌中的作用

• 批准号: 6342211
• 负责人: STEVE NORDEEN
• 金额: $ 33.37万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6342211
• 关键词: SCID mouse; adenocarcinoma; androgens; antisense nucleic acid; athymic mouse; cadherins; cell motility; complementary DNA; fibroblast growth factor; gene expression; homeobox genes; hormone regulation /control mechanism; human tissue; immunocytochemistry; integrins; metastasis; molecular cloning; neoplasm /cancer genetics; neoplastic growth; northern blottings; nucleic acid sequence; polymerase chain reaction; prostate neoplasms; tumor suppressor genes; western blottings

Our role in this collaboration will be the construction and biological characterization of AT6.1-12 microcell hybrids that contain the HOXC gene locus. The Miller laboratory will then analyze HOXC gene expression patterns in these hybrids to see if there is a correlation between HOXC gene expression and in vivo biological activity. Once candidate HOXC genes have been identified, our laboratory will test their potential biological activity by transfecting them into AT6.1 cells, establishing appropriate transgene and vector only controls, and finally testing these cell lines in vivo.

我们在此次合作中的角色将是包含 HOXC 基因位点的 AT6.1-12 微细胞杂交体的构建和生物学表征。米勒实验室随后将分析这些杂交体中的 HOXC 基因表达模式，看看 HOXC 基因表达与体内生物活性之间是否存在相关性。一旦确定了候选 HOXC 基因，我们的实验室将通过将其转染至 AT6.1 细胞、建立适当的转基因和仅载体对照来测试其潜在的生物活性，并最终在体内测试这些细胞系。