Sampler for viable airborne bacteria, fungi and virus

空气中活细菌、真菌和病毒采样器

• 批准号: 6617634
• 负责人: IGOR E AGRANOVSKI
• 金额: $ 10.68万
• 依托单位: GRIFFITH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6617634
• 关键词: Fungi; air sampling /monitoring; bacteria; bioengineering /biomedical engineering; biohazard detection; biomedical equipment development; bioterrorism /chemical warfare; microbiology; microorganism; polymerase chain reaction; portable biomedical equipment; sample collection; virus

DESCRIPTION (provided by applicant): A new method for removal of airborne particles from air by bubbling through porous medium immersed into liquid has been developed and shown to be efficient for cleaning environments with ultra-fine particles. Some studies have demonstrated that the new technique can be suitable for collecting viable airborne microorganisms even if they are sensitive to stress. The principal objective of the present study at the initial stage of investigation (R21) is to evaluate the new bubbling technique for collection of viable airborne microorganisms, including viruses, bacteria and fungi into appropriate liquids for subsequent physical and microbiological analysis. The main expected outcomes of the entire study (R21/R33) will be a new methodology and a fully functional prototype of equipment for representative personal sampling of viable airborne microorganisms. Accurate and reliable information about concentration of pathogenic viable airborne microorganisms in ambient air is one of the most important issues in biomedical research for evaluation of health effects. Other potential users of the device are security and antiterrorist units that deal with the detection and quantification of airborne biological agents. The main aims will be achieved through extensive laboratory research together with field evaluation of the new equipment. The laboratory research will be conducted by using most advanced equipment available for the bioaerosol evaluation including monitoring of physical collection efficiency of the sampler by Scanning Mobility Particle Sizer (TSI, USA) and Laser Particle Counter. Microorganism recovery rates will be identified by using culturing technique for bacteria and fungi and by virus titration technique for viruses. Also, nature and total amount of particular microorganisms of interest collected during field studies will be identified by standard PCR technique (DNA microorganisms) and RT - PCR (RNA microorganisms). Field evaluation will be performed in different environments including low concentrated areas (offices and residential buildings) and highly concentrated areas (agriculture and waste management facilities). The new personal sampler will become a first available technique suitable for monitoring viable airborne viruses in an ambient air and first personal sampling technique capable to provide eight hours non-stop operation.

描述（由申请人提供）： 一种通过浸入液体中的多孔介质鼓泡来去除空气中悬浮颗粒的新方法已经被开发出来，并且被证明可以有效地清洁含有超细颗粒的环境。 一些研究表明，新技术适用于收集活的空气传播微生物，即使它们对压力敏感。 本研究在调查初始阶段（R21）的主要目标是评估新的鼓泡技术，用于将活的空气传播微生物（包括病毒、细菌和真菌）收集到适当的液体中，以便进行后续的物理和微生物分析。 整个研究（R21/R33）的主要预期成果将是一种新的方法和功能齐全的设备原型，用于对活的空气微生物进行代表性的个人采样。 有关环境空气中致病性活空气传播微生物浓度的准确可靠的信息是评估健康影响的生物医学研究中最重要的问题之一。 该设备的其他潜在用户是负责检测和量化空气中生物制剂的安全和反恐单位。 主要目标将通过广泛的实验室研究以及新设备的现场评估来实现。 实验室研究将使用可用于生物气溶胶评估的最先进设备进行，包括通过扫描移动粒度仪（TSI，美国）和激光粒子计数器监测采样器的物理收集效率。 微生物回收率将通过细菌和真菌的培养技术以及病毒的病毒滴定技术来确定。 此外，在现场研究期间收集的特定感兴趣微生物的性质和总量将通过标准 PCR 技术（DNA 微生物）和 RT - PCR（RNA 微生物）进行鉴定。 现场评估将在不同的环境中进行，包括低集中区域（办公楼和住宅楼）和高集中区域（农业和废物管理设施）。 新的个人采样器将成为第一个适用于监测环境空气中活的空气传播病毒的可用技术，也是第一个能够提供八小时不间断运行的个人采样技术。