CORE--ANIMAL

核心--动物

• 批准号: 6663971
• 负责人: DENNIS B. LEEPER
• 金额: $ 22.84万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6663971
• 关键词: SCID mouse; animal care; animal colony; biomedical facility; human tissue; melanoma; neoplastic cell; xenotransplantation

The Animal Care Core will provide animal care, animal housing and preparation of SCID mice and xenograft tumors for experimentation. The core activity is also responsible for coordinating the administration, regulatory reporting and fiscal management of animal care activities. Since SCID mice are immune suppressed and contain human tumors, specialized care is required. All animal procedures require sterile technique and handlers require specialized training. The Animal Care Core staff orders SCID mice, maintain the daily records on all of the tumors and animals, inject explanted human melanoma cells into recipient xenografts, monitor the growth of primary tumor xenografts in the F1 and F2 generations, transplant F2 tumors as required for treatment and prepare xenografts for -95 degrees Celsius storage. The Animal Core staff is responsible for preparation of all regulatory records for the Department of Animal Resources and the I.A.C.U.C. The routine daily maintenance, husbandry and facility hygiene for the animals are provided as a purchased service by the Department of Animal Resources. Animals are initially housed in isolation in the Central Animal Facility, tumors inoculated and transplanted in the Central Animal Facility under Biosafety Level 2 conditions and then transferred under sterile conditions to the laboratory animal facility for monitoring tumor growth for subsequent treatment in Projects 1, 2, 3 or 4. Dennis B. Leeper is the Core Director and Judith A. Daviau, Institute Veterinarian, is the Co-Director. Dr. Leeper will direct and oversee all operations. Dr. Daviau will have a critical role in monitoring all operations, reviewing all procedures, seeing to the health and care of all animals and helping improve and upgrade animal care and surgical procedures. Dr. Daviau's specialty is the care of laboratory animals. Both Dr. Daviau and Dr. Leeper have extensive experience supervising animal care, including colonies of SCID mice. Dr. Leeper is a member of the I.A.C.U.C. The Animal Care Core provides explanted melanoma cells from xenografts for Projects 1,2 and 3 and provides all the animals and tumors for Project 4 and the Subcontract with the University of Pennsylvania. The laboratory animal facility is A.A.A.L.A.C. approved.

动物护理核心将提供动物护理，动物外壳和 制备SCID小鼠和异种移植肿瘤进行实验。这 核心活动还负责协调管理， 动物护理活动的监管报告和财政管理。 由于SCID小鼠被免疫抑制并含有人类肿瘤，因此 需要专业护理。所有动物程序都需要无菌 技术和处理程序需要专门的培训。 动物护理核心人员命令SCID小鼠，维护每日记录 所有的肿瘤和动物，都注入植物的人类黑色素瘤细胞 接受者异种移植物，监测原发性肿瘤异种移植物在 F1和F2代，治疗所需的移植F2肿瘤 并准备-95摄氏度存储的异种移植物。动物核心 员工负责为所有监管记录做准备 动物资源部和I.A.C.U.C.日常工作 为动物提供维护，饲养和设施卫生 作为动物资源部购买的服务。动物是 肿瘤最初孤立地安置在中央动物设施中 在生物安全下接种并移植在中央动物设施中 2级条件，然后在无菌条件下转移到 用于监测肿瘤生长的实验室动物设施 项目1、2、3或4中的处理。 丹尼斯·B·莱珀（Dennis B. 兽医是联合导演。 Leeper博士将指导并监督所有 运营。 Daviau博士将在监视所有方面发挥关键作用 操作，审查所有程序，了解所有人的健康和护理 动物并帮助改善和升级动物护理和手术 程序。 Daviau博士的专长是对实验动物的护理。两个都 Daviau博士和Leeper博士在监督动物方面拥有丰富的经验 护理，包括SCID小鼠的殖民地。 Leeper博士是 I.A.C.U.C.动物护理核心提供了来自 项目1,2和3的异种移植物，并提供所有动物和肿瘤 对于项目4和宾夕法尼亚大学的分包合同。 实验室动物设施为A.A.A.L.A.C.得到正式认可的。