CORE--ANIMAL

核心--动物

• 批准号: 6269552
• 负责人: DENNIS B. LEEPER
• 金额: $ 14.43万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6269552
• 关键词: SCID mouse; animal care; animal colony; biomedical facility; human tissue; melanoma; neoplastic cell; xenotransplantation

The Animal Care Core will provide animal care, animal housing and preparation of SCID mice and xenograft tumors for experimentation. The core activity is also responsible for coordinating the administration, regulatory reporting and fiscal management of animal care activities. Since SCID mice are immune suppressed and contain human tumors, specialized care is required. All animal procedures require sterile technique and handlers require specialized training. The Animal Care Core staff orders SCID mice, maintain the daily records on all of the tumors and animals, inject explanted human melanoma cells into recipient xenografts, monitor the growth of primary tumor xenografts in the F1 and F2 generations, transplant F2 tumors as required for treatment and prepare xenografts for -95 degrees Celsius storage. The Animal Core staff is responsible for preparation of all regulatory records for the Department of Animal Resources and the I.A.C.U.C. The routine daily maintenance, husbandry and facility hygiene for the animals are provided as a purchased service by the Department of Animal Resources. Animals are initially housed in isolation in the Central Animal Facility, tumors inoculated and transplanted in the Central Animal Facility under Biosafety Level 2 conditions and then transferred under sterile conditions to the laboratory animal facility for monitoring tumor growth for subsequent treatment in Projects 1, 2, 3 or 4. Dennis B. Leeper is the Core Director and Judith A. Daviau, Institute Veterinarian, is the Co-Director. Dr. Leeper will direct and oversee all operations. Dr. Daviau will have a critical role in monitoring all operations, reviewing all procedures, seeing to the health and care of all animals and helping improve and upgrade animal care and surgical procedures. Dr. Daviau's specialty is the care of laboratory animals. Both Dr. Daviau and Dr. Leeper have extensive experience supervising animal care, including colonies of SCID mice. Dr. Leeper is a member of the I.A.C.U.C. The Animal Care Core provides explanted melanoma cells from xenografts for Projects 1,2 and 3 and provides all the animals and tumors for Project 4 and the Subcontract with the University of Pennsylvania. The laboratory animal facility is A.A.A.L.A.C. approved.

动物护理核心将提供动物护理、动物住房和 制备SCID小鼠和异种移植肿瘤用于实验。这 核心活动还负责协调行政管理， 动物护理活动的监管报告和财务管理。 由于 SCID 小鼠受到免疫抑制并含有人类肿瘤， 需要专门护理。所有动物程序都需要无菌 技术和操作人员需要专门培训。 动物护理核心人员订购 SCID 小鼠，维护每日记录 所有的肿瘤和动物，将移植的人类黑色素瘤细胞注射到 受体异种移植物，监测原发肿瘤异种移植物的生长 F1和F2代，根据治疗需要移植F2肿瘤 并准备异种移植物用于-95摄氏度储存。动物核心 工作人员负责准备所有监管记录 动物资源部和 I.A.C.U.C.日常的日常 为动物提供维护、饲养和设施卫生 作为动物资源部购买的服务。动物是 最初被隔离在中央动物设施中，肿瘤 在生物安全的中央动物设施中接种和移植 2级条件，然后在无菌条件下转移至 用于监测肿瘤生长以用于后续研究的实验动物设施 项目 1、2、3 或 4 中的处理。 Dennis B. Leeper 是研究所的核心主任，Judith A. Daviau 是研究所的核心主任 兽医，是联合主任。 Leeper 博士将指导和监督所有 运营。达维奥博士将在监测所有 运营，审查所有程序，照顾所有人的健康和护理 动物并帮助改善和升级动物护理和外科手术 程序。 Daviau 博士的专长是实验动物的护理。两个都 Daviau 博士和 Leeper 博士拥有丰富的动物监管经验 护理，包括 SCID 小鼠群体。 Leeper 博士是该组织的成员 I.A.C.U.C.动物护理核心提供来自以下组织的外植黑色素瘤细胞： 项目 1,2 和 3 的异种移植物并提供所有动物和肿瘤 项目 4 以及与宾夕法尼亚大学的分包合同。 实验动物设施是 A.A.A.L.A.C.得到正式认可的。