CORE--PREPARATION OF BIOLOGICAL SAMPLES

核心--生物样品的制备

• 批准号: 6602697
• 负责人: ELEANOR G ROGAN
• 金额: $ 16.87万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6602697
• 关键词: biomedical facility; breast neoplasms; chemical registry /resource; human tissue; prostate neoplasms; tissue resource /registry

Continuation of the Core component is proposed to serve as a centralized source of estrogen compounds, linker conjugates, treated and non-treated oligonucleotides and mouse skin treated with polycyclic aromatic hydrocarbons (PAH), and as a repository for all human tissue and urine samples to be used in all five research project. All of the necessary facilities and expertise for the Core are available in the Cavelieri-Rogan research groups or elsewhere in the Eppley Institute. In brief, the Core will provide catechol estrogens (CE), CE-Q, depurinating CE-DNA adducts and CE conjugates with glutathione (and its breakdown products in the mercapturic acid biosynthesis pathway, namely, cysteine and N- acetylcysteine) to Projects 1-5 as needed for experiments or as reference standards enzyme assays or analytical techniques.. The Core will also provide linker conjugates for development of monoclonal antibodies to Project 4. The Core will provide various oligonucleotides, either treated with PAH or CE-Q, or non-treated to Projects 3 and 5. In addition, the Core will serve as the repository for all human breast, prostate tissue and urine samples, procured at UNMC, the Mayo Clinic or NYU School of Medicine, and distribute them to Projects 1 and 2 as needed. The Core will provide high quality synthesized estrogen compounds for the five projects and is essential for he preparation of linker conjugates used in Project 4. More important will be the procurement, storage, cataloguing and distribution of human samples for the study of breast cancer (tissue and urine samples from UNMC and Mayo) and prostate cancer (tissue and urine samples from NYU).

提出核心成分的延续作为雌激素化合物，接头偶联物，处理和未经处理的寡核苷酸以及用多环芳族烃（PAH）处理的小鼠皮肤的集中式来源在所有五个研究项目中都使用。 Cavelieri-Rogan研究小组或Eppley Institute的其他地方都可以找到核心的所有必要设施和专业知识。简而言之，核心将提供儿茶酚雌激素（CE），CE-Q，降低Ce-DNA加合物和CE偶联物与谷胱甘肽（及其在苏维他酸生物合成途径中的分解产物，即，CySteine和N-乙酰囊这氨酸）对1个项目1 -5根据实验或作为参考标准的酶测定或分析技术的需要。核心还将为项目4的单克隆抗体开发开发接头。未对项目3和5进行处理。此外，该核心将作为所有人类乳房，前列腺组织和尿液样本的存储库，在UNMC，Mayo诊所或纽约大学医学院采购，并将其分配给项目1和项目1和项目1和项目。 2根据需要。核心将为五个项目提供高质量的合成雌激素化合物，对于项目4中使用的接头缀合物至关重要。更重要的是，人类样品的采购，存储，分类和分布进行乳腺癌研究（组织）来自UNMC和Mayo）和前列腺癌（来自纽约大学的组织和尿液样本）的尿液样品。