CORE--BREAST CANCER CELL LINE RESOURCE

核心--乳腺癌细胞系资源

基本信息

批准号：
6397864
负责人：
BARBARA L ZIFF
金额：
$ 13.53万
依托单位：
GEORGETOWN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-09-16 至 2003-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6397864
关键词：
Mycoplasma bioassay biomedical facility breast neoplasms charcoal chemical fingerprinting clone cells genetic transduction growth media helper virus plasmids tissue /cell culture tissue resource /registry transfection transfection /expression vector

项目摘要

The primary functions of this core facility are to manage the breast cancer cell line repository and to provide essential tissue culture support to breast cancer researchers. The facility provides shared tissue culture areas that are well equipped and stocked with all of the necessary tissue culture reagents and supplies. Breast cancer cell lines are cultured and made available to investigators as either growing or frozen cell stocks. Frozen cell stocks are replenished as required. DNA fingerprinting of cell lines is used as a quality control measure. The breast cancer cell line repository is managed through a complete inventory system. The individual cell lines of the repository are described in a computer database that is part of the SPORE computer network. A number of tissue culture related services are offered, including mycoplasma testing, helper virus rescue assays, charcoal stripping of calf serum, cell culture, cell freezing, and growth curve analysis of new lots of both calf serum and fetal calf serum. The production of primary cell lines from normal breast tissue and from breast cancer primary or metastatic tumors is a developing service. The shared tissue culture areas are used by the investigators, trainees and technicians from Il LCRC breast cancer research laboratories who do not have their own individual tissue culture space. The breast cancer cell line repository is used by 13 breast cancer research laboratories. All of the breast cancer research laboratories regularly request mycoplasma testing. The helper virus rescue assay has been used by four breast cancer research laboratories. Charcoal stripped calf serum produced in the core facility is used by seven breast cancer research laboratories. The cell culture service has been used by eight breast cancer research laboratories.

该核心设施的主要功能是管理乳房癌细胞系存储库并提供必需的组织培养支持乳腺癌研究人员。该设施提供共享组织装备良好且库存的文化领域必要的组织培养试剂和供应。乳腺癌细胞系被培养并提供给调查人员，因为成长或冷冻的细胞库存。根据需要补充冷冻的细胞库存。脱氧核糖核酸细胞系的指纹用作质量控制措施。这乳腺癌细胞系存储库是通过完整的库存系统。存储库的单个单元线是在计算机数据库中描述，该数据库是孢子计算机的一部分网络。提供许多与组织培养相关的服务，包括支原体测试，辅助病毒救援测定法，木炭小牛血清的剥离，细胞培养，细胞冷冻和生长曲线分析新的小牛血清和胎儿小腿血清。这从正常乳腺组织和乳腺癌原发性或转移性肿瘤是一种发展服务。研究人员，学员使用共享组织培养区域以及IL LCRC乳腺癌研究实验室的技术人员没有自己的单个组织培养空间。乳腺癌 13个乳腺癌研究实验室使用细胞系存储库。所有乳腺癌研究实验室定期要求支原体测试。辅助病毒救援测定法已被四个乳腺癌研究实验室。木炭剥离的小腿精华素在核心设施中生产的七个乳腺癌研究使用实验室。细胞培养服务已被八个乳房使用癌症研究实验室。