CORE--PREPARATION OF BIOLOGICAL SAMPLES

核心--生物样品的制备

• 批准号: 6102530
• 负责人: ELEANOR G ROGAN
• 金额: $ 16.87万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6102530
• 关键词: animal genetic material tag; biomedical facility; chemical registry /resource; laboratory mouse; tissue /cell culture; tissue resource /registry

The core represents the most efficient, cost-effective, reliable source of the required samples, which will range from modified oligonucleotides and larger DNA sequences to mouse skin treated with polycyclic aromatic hydrocarbons (PAH) and mouse papillomas. All of th necessary facilities and expertise for the Core are available in the Eppley Institute. In brief, the Core will provide mouse skin treated with PA, cells from mouse skin treated with PAH, HeLa cells treated with PAH, and mouse skin papillomas induced by PAH. In addition, the Core will provide 18-base pair oligonucleotides either containing an apurinic site or modified with PAH or catechol estrogen quinones (CE-Q) and longer defined DNA sequences modified with PAH or CE-Q. Provision of biological preparations by the Core component will expedite the research conducted in the program project grant. In addition, analysis of split samples by collaborators will enhance quality control and will assure that comparison of results obtained by different methods is valid.

核心是最有效，最具成本效益，可靠的来源 所需的样品，其范围从修改的寡核苷酸和 用多环芳烃处理的小鼠皮肤的较大DNA序列 碳氢化合物（PAH）和小鼠乳头状瘤。 所有必要的设施 Eppley Institute提供了核心的专业知识。 在 简而 用PAH处理的皮肤，用PAH处理的HeLa细胞和小鼠皮肤 PAH诱导的乳头状瘤。 此外，核心将提供18垒对 寡核苷酸含有载膜位点或用PAH修饰或 Catechol雌激素喹酮（CE-Q）和更长的DNA序列修饰 与PAH或CE-Q。 核心提供生物制剂 组件将加快计划项目中的研究 授予。 此外，合作者对拆分样本的分析将 增强质量控制，并确保比较获得的结果 通过不同的方法是有效的。