CORE--SCIENTIFIC

核心--科学

• 批准号: 6668220
• 负责人: Patricia Ann D'Amore
• 金额: $ 9.16万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6668220
• 关键词: angiogenesis; bioimaging /biomedical imaging; biomedical facility; chick embryo; confocal scanning microscopy; histology; image processing; immunocytochemistry; photography; tissue /cell culture; tissue /cell preparation; vascular endothelium

The goal of the Scientific Core is to supportand facilitate the research programs of the investigators in the program project. The overall Scientific Core consists of a Photography Sub-Core, an Angiogenesis Assay Sub-Core,andan imaging Sub-Core. (i) The Photography Core provides high quality, in-house conventional and digital services, including figures for publication, and imagesfor slide and Power Point presentations. This Core is staffed by one full-time photographer with over3 years experience in the department and a parttime assistant. (ii) The Angiogenesis Assay Core will provide microvascular endothelial cells and expertise in carrying out angiogenesis assays, including chick aortic rings to measure angiogenesis in vitro, and the chick chorioallantoic membrane assay to test angiogenesis promoters and inhibitors in vivo. This Core is staffed by two senior technicians, each with over 15 years experience, one in large and small vessel endothelial cell preparation and biology, and the other in the developing chick embryo system. (iii)The imaging Core is designed to transform the original histology core into a modem high-resolution operation. This Core will provide a centralized facility for taser-confocal microscopy, real-time imaging, and three-dimensional reconstruction of living ceils, whole embryos, and histological sections of tumors and developing vascular tissue. The Imaging Core will be staffed by an experienced confocal microscopist who will operate the shared laser-scanning confocal microscope, instruct and assist Project members in its use, and manage access to the facility. This new facility is critical to a number of the specific aims of the Program Project.

科学核心的目标是支持和促进项目中研究者的研究项目。整个科学核心由摄影子核心、血管生成测定子核心和成像子核心组成。 (i) 摄影核心提供高质量的内部传统和数字服务，包括用于出版的图片以及用于幻灯片和 Power Point 演示的图像。该核心人员由一名具有3年以上部门经验的全职摄影师和一名兼职助理组成。 (ii) 血管生成检测核心将提供微血管内皮细胞和进行血管生成检测的专业知识，包括用于体外测量血管生成的鸡主动脉环，以及用于测试体内血管生成促进剂和抑制剂的鸡绒毛尿囊膜检测。该核心由两名高级技术人员组成，每名技术人员均拥有超过15年的经验，其中一名在大小血管内皮细胞制备和生物学方面，另一名在发育中的鸡胚胎系统方面。 (iii)成像核心旨在改变原始组织学 核心变成调制解调器高分辨率操作。该核心将为泰瑟枪共聚焦显微镜、实时成像以及活细胞、整个胚胎以及肿瘤和发育中血管组织的组织学切片的三维重建提供集中设施。成像核心将配备一名经验丰富的共焦显微镜专家，他将操作共享的激光扫描共焦显微镜，指导和协助项目成员使用该显微镜，并管理对该设施的访问。 这个新设施对于该计划项目的许多具体目标至关重要。