Transmission Electron Microscope

透射电子显微镜

• 批准号: 8052990
• 负责人: Patricia Ann D'Amore
• 金额: $ 43.39万
• 依托单位: SCHEPENS EYE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052990
• 关键词: Accounting; Advisory Committees; Cells; Computers; Contract Services; Contracts; Data; Electron Microscope; Electrons; Elements; Equipment; Eye; Fee-for-Service Plans; Fees and Charges; Funding; Funding Applicant; Guns; Head; Histology; Image; Imagery; Institution; Letters; Maintenance; Mediation; Membrane Biology; Microscope; Microtome - medical device; Minor; Morphology; Nanotechnology; Optics; Principal Investigator; Research; Research Institute; Sampling; Services; Staging; Staging System; Staining method; Stains; Three-Dimensional Imaging; Time; Tissues; Vacuum; Work; base; charge coupled device camera; cost; discount; instrument; lens; novel; operation; programs; tissue preparation; tomography; transmission process

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Tecnai G2 Spirit BioTWIN G2 transmission electron microscope (TEM) and microtome. This TEM is a state-of-the-art instrument and contains a high-contrast BioTWIN objective lens optimized for 2D and 3D imaging of stained or unstained, low-contrast samples. All of the microscope components including the electron gun, the optical elements, the vacuum system and the stage, are digitally controlled, allowing the user to automatically recall all optimized operating conditions including lens settings, gun parameters, optical alignments, aperture alignments. Similarly, the CCD camera is computer controlled so that image acquisition can be automated and connected to a network for exporting data. Four major users and seven minor users, whose work is supported by 16 RO1's, and 3 R21's, 1 PO1 and 1 DP2, will utilize the microscope. These users will account for approximately 32 hr/week (80% usage of the microscope time). The remainder of the time will be available for new users at Schepens Eye Research Institute (SERI) and, when available, for outside users. The requested TEM will replace the current a Phillips 41OLS, which is 25 years old and for which replacement parts are no longer available. The Tecnai G2 Spirit BioTWIN G2 lists at $659,492 but we have negotiated a very significant discount and have a quote to purchase the scope, camera and tomography stage at $600,000. The high internal usage of the scope makes it infeasible to use services at neighboring institutions. The scope will be integrated into the Morphology Unit and will be overseen and operated by Ms. Patricia Pearson, who has been an integral part of our Morphology Unit for over 30 years and is recognized as an expert in the field of ocular histology. A fulltime research technician, supported by SERI, who will be responsible for tissue preparation and sectioning, will assist Ms. Pearson. Work on the TEM will be carried out as a fee-for-service, with work done on a first come-first serve basis. The service contract will be with Nanotech, who specializes in the service of Philips and FEI electron microscopes, and who has been servicing our current scope for more than 20 years. The cost of the contract is built into the fees that are charged for the TEM services. SERI also maintains a fund for the service and maintenance of common equipment and these funds will be available for expenses not covered in any contract, as was done with the Phillips 41OLS. An internal advisory committee will assist the Principal Investigator with maximizing the utilization and overseeing the day-to-day management of the microscope as well as with establishing a financial plan for the long-term operation and maintenance of the microscope. Dr. Dennis Brown who heads the Imaging Core for the Mass General Program in Membrane Biology has been added to the advisory committee and will be available for advice on novel applications such as tomography. Institutional commitment is evidenced in the included letter from Dr. Eli Peli, a Co- Director of Research at SERI. As more and more of our research has come to rely on ultrastructural visualization of cells and tissues, demand for the TEM has grown and access to this state of the art TEM will undoubtedly facilitate and support our work. ) PUBLIC HEALTH RELEVANCE: Transmission electron microscopy (TEM) permits the visualization of cells and tissues at the subcellular level by providing very high magnification. There has been an increasing utilization of imaging technologies to understand the structure/function of genes of interest. The requested TEM will be used to examine how certain genes and proteins are involved in normal visual processes and how they are altered in diseased tissues of the eye.

描述（由申请人提供）：要求购买Tecnai G2 Spirit Biotwin G2传输电子显微镜（TEM）和Microtome的资金。该TEM是一种最先进的仪器，包含用于优化的高对比度Biotwin物镜，可针对染色或未染色的低对比度样品的2D和3D成像进行优化。所有显微镜组件包括电子枪，光学元素，真空系统和阶段，都受到数字控制，使用户可以自动回忆所有优化的操作条件，包括镜头设置，枪支参数，光学对齐，光圈对准。同样，CCD摄像机受到计算机的控制，因此可以自动化图像采集并连接到网络以导出数据。四个主要用户和7个次要用户，他们的工作得到了16个RO1的支持，其中3个R21，1个PO1和1 DP2将使用显微镜。这些用户将占约32小时/周（显微镜时间使用80％）。 Schepens Eye Research Institute（SERI）的新用户以及外部用户的剩余时间将为新用户提供。请求的TEM将替换电流A Phillips 41ols，该phillips 41ols已有25年的历史，并且不再可用。 Tecnai G2 Spirit Biotwin G2的价格为659,492美元，但我们已经谈判了非常明显的折扣，并有报价以购买范围，相机和断层扫描阶段的报价为60万美元。范围内部使用的高度使用使在附近的机构使用服务是不可行的。该范围将集成到形态学单元中，并将由Patricia Pearson女士监督和经营，Patricia Pearson女士已成为我们形态学单位不可或缺的一部分已有30多年的历史，并被公认为是眼组织学领域的专家。由Seri支持的全职研究技术员，将负责组织准备和切片，将协助Pearson女士。 TEM上的工作将以支付费用进行，并以第一次发球的基础进行工作。该服务合同将与Nanotech进行服务，Nanotech专门从事飞利浦和FEI电子显微镜服务，并且已经为我们当前的范围服务了20多年。合同的成本内置在用于TEM服务的费用中。 SERI还维护了一项用于通用设备的服务和维护的资金，这些资金将用于任何合同中未支付的费用，就像Phillips 41ols所做的那样。内部咨询委员会将协助首席研究人员最大程度地利用并监督显微镜的日常管理，并建立用于长期操作和维护显微镜的财务计划。丹尼斯·布朗（Dennis Brown）博士负责膜生物学大众一般计划的成像核心，已添加到咨询委员会中，并将提供有关诸如层析成像等新型应用的建议。 Seri研究联合主任Eli Peli博士的纳入信中证明了机构承诺。随着我们越来越多的研究依靠细胞和组织的超微结构可视化，对TEM的需求已经越来越不断增长，并且可以使用这种最新的TEM，无疑将有助于和支持我们的工作。 ） 公共卫生相关性：传播电子显微镜（TEM）允许通过提供非常高的放大倍率在亚细胞水平上可视化细胞和组织。成像技术的利用越来越多，以了解感兴趣基因的结构/功能。请求的TEM将用于检查某些基因和蛋白质如何参与正常的视觉过程，以及它们如何在眼睛的患病组织中改变它们。