Transmission Electron Microscope

透射电子显微镜

• 批准号: 8052990
• 负责人: Patricia Ann D'Amore
• 金额: $ 43.39万
• 依托单位: SCHEPENS EYE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052990
• 关键词: Accounting; Advisory Committees; Cells; Computers; Contract Services; Contracts; Data; Electron Microscope; Electrons; Elements; Equipment; Eye; Fee-for-Service Plans; Fees and Charges; Funding; Funding Applicant; Guns; Head; Histology; Image; Imagery; Institution; Letters; Maintenance; Mediation; Membrane Biology; Microscope; Microtome - medical device; Minor; Morphology; Nanotechnology; Optics; Principal Investigator; Research; Research Institute; Sampling; Services; Staging; Staging System; Staining method; Stains; Three-Dimensional Imaging; Time; Tissues; Vacuum; Work; base; charge coupled device camera; cost; discount; instrument; lens; novel; operation; programs; tissue preparation; tomography; transmission process

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Tecnai G2 Spirit BioTWIN G2 transmission electron microscope (TEM) and microtome. This TEM is a state-of-the-art instrument and contains a high-contrast BioTWIN objective lens optimized for 2D and 3D imaging of stained or unstained, low-contrast samples. All of the microscope components including the electron gun, the optical elements, the vacuum system and the stage, are digitally controlled, allowing the user to automatically recall all optimized operating conditions including lens settings, gun parameters, optical alignments, aperture alignments. Similarly, the CCD camera is computer controlled so that image acquisition can be automated and connected to a network for exporting data. Four major users and seven minor users, whose work is supported by 16 RO1's, and 3 R21's, 1 PO1 and 1 DP2, will utilize the microscope. These users will account for approximately 32 hr/week (80% usage of the microscope time). The remainder of the time will be available for new users at Schepens Eye Research Institute (SERI) and, when available, for outside users. The requested TEM will replace the current a Phillips 41OLS, which is 25 years old and for which replacement parts are no longer available. The Tecnai G2 Spirit BioTWIN G2 lists at $659,492 but we have negotiated a very significant discount and have a quote to purchase the scope, camera and tomography stage at $600,000. The high internal usage of the scope makes it infeasible to use services at neighboring institutions. The scope will be integrated into the Morphology Unit and will be overseen and operated by Ms. Patricia Pearson, who has been an integral part of our Morphology Unit for over 30 years and is recognized as an expert in the field of ocular histology. A fulltime research technician, supported by SERI, who will be responsible for tissue preparation and sectioning, will assist Ms. Pearson. Work on the TEM will be carried out as a fee-for-service, with work done on a first come-first serve basis. The service contract will be with Nanotech, who specializes in the service of Philips and FEI electron microscopes, and who has been servicing our current scope for more than 20 years. The cost of the contract is built into the fees that are charged for the TEM services. SERI also maintains a fund for the service and maintenance of common equipment and these funds will be available for expenses not covered in any contract, as was done with the Phillips 41OLS. An internal advisory committee will assist the Principal Investigator with maximizing the utilization and overseeing the day-to-day management of the microscope as well as with establishing a financial plan for the long-term operation and maintenance of the microscope. Dr. Dennis Brown who heads the Imaging Core for the Mass General Program in Membrane Biology has been added to the advisory committee and will be available for advice on novel applications such as tomography. Institutional commitment is evidenced in the included letter from Dr. Eli Peli, a Co- Director of Research at SERI. As more and more of our research has come to rely on ultrastructural visualization of cells and tissues, demand for the TEM has grown and access to this state of the art TEM will undoubtedly facilitate and support our work. ) PUBLIC HEALTH RELEVANCE: Transmission electron microscopy (TEM) permits the visualization of cells and tissues at the subcellular level by providing very high magnification. There has been an increasing utilization of imaging technologies to understand the structure/function of genes of interest. The requested TEM will be used to examine how certain genes and proteins are involved in normal visual processes and how they are altered in diseased tissues of the eye.

描述（由申请人提供）： 需要资金购买 Tecnai G2 Spirit BioTWIN G2 透射电子显微镜 (TEM) 和切片机。该 TEM 是一款最先进的仪器，包含一个高对比度 BioTWIN 物镜，针对染色或未染色、低对比度样品的 2D 和 3D 成像进行了优化。所有显微镜组件（包括电子枪、光学元件、真空系统和载物台）均采用数字控制，使用户能够自动调用所有优化的操作条件，包括镜头设置、电子枪参数、光学对准、孔径对准。同样，CCD 相机由计算机控制，因此可以自动采集图像并连接到网络以导出数据。四个主要用户和七个次要用户将使用该显微镜，他们的工作由 16 个 RO1、3 个 R21、1 个 PO1 和 1 个 DP2 支持。这些用户每周大约使用 32 小时（使用显微镜时间的 80%）。剩余时间将供舍彭斯眼科研究所 (SERI) 的新用户使用，并且在可用时也供外部用户使用。所请求的 TEM 将取代当前的 Phillips 41OLS，该型号已有 25 年历史，且不再提供替换零件。 Tecnai G2 Spirit BioTWIN G2 的售价为 659,492 美元，但我们已经协商了非常大的折扣，并以 600,000 美元的价格购买示波器、相机和断层扫描平台的报价。该范围的高内部使用率使得无法使用邻近机构的服务。该示波器将并入形态学部门，并由 Patricia Pearson 女士监督和操作。30 多年来，她一直是我们形态学部门不可或缺的一部分，被公认为眼组织学领域的专家。由 SERI 支持的一名全职研究技术人员将负责组织准备和切片，并协助 Pearson 女士。 TEM 的工作将按服务收费的方式进行，工作按照先到先得的原则进行。该服务合同将与 Nanotech 签订，该公司专门从事飞利浦和 FEI 电子显微镜的服务，并且为我们现有的显微镜提供服务已超过 20 年。合同费用计入 TEM 服务的费用中。 SERI 还设立了一项用于通用设备服务和维护的基金，这些基金将可用于支付任何合同中未涵盖的费用，就像 Phillips 41OLS 所做的那样。内部咨询委员会将协助首席研究员最大限度地利用和监督显微镜的日常管理，以及制定显微镜长期运行和维护的财务计划。膜生物学大众通用计划成像核心负责人 Dennis Brown 博士已被添加到咨询委员会中，并将就断层扫描等新颖应用提供建议。 SERI 联合研究主任 Eli Peli 博士的信函证明了机构的承诺。随着我们越来越多的研究依赖于细胞和组织的超微结构可视化，对 TEM 的需求不断增长，而使用这种最先进的 TEM 无疑将促进和支持我们的工作。 ） 公共健康相关性：透射电子显微镜 (TEM) 通过提供非常高的放大倍数，可以在亚细胞水平上观察细胞和组织。人们越来越多地利用成像技术来了解感兴趣基因的结构/功能。所要求的 TEM 将用于检查某些基因和蛋白质如何参与正常视觉过程以及它们在眼睛患病组织中如何改变。