Piezoelectric Pipetting Technology for DNA Analysis

用于 DNA 分析的压电移液技术

• 批准号: 6618819
• 负责人: PETER J. WIKTOR
• 金额: $ 53.49万
• 依托单位: ENGINEERING ARTS
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6618819
• 关键词: DNA; biomedical automation; biomedical equipment development; computer program /software; computer system design /evaluation; computer system hardware; fluid flow; gene expression; genetic polymorphism; genotype; microarray technology; nucleic acid hybridization; nucleic acid quantitation /detection; polymerase chain reaction; technology /technique development

DESCRIPTION (provided by applicant): The proposed research follows two distinct yet complementary paths. One path involves developing and testing novel piezoelectric pipetting, dispensing, sensing and housing technology and integrating this into a fully automated piezoelectric pipetting system. Innovative piezoelectric-based technology enables pipetting of sub-nanoliter volumes of fluid and continuous monitoring to detect clogged tips and other operational states. The other path involves doing cancer-related genomics research in collaborative studies with the Fred Hutchinson Cancer Research Center (F.H.C.R.C.) to use the proposed piezoelectric pipetting system to 1) develop more robust oligonucleotide or cDNA-based microarray platforms for gene expression analyses, 2) complete the development of a cDNA microarray-based platform for simultaneous genotyping and gene expression monitoring and 3) develop novel, high-throughput DNA microarray-based assays of cellular function that will make it possible to assay phenotypic effects of silencing specific genes on a genomic scale. On the one hand, the molecular-biology research provides a real-world application to help focus the design of the piezoelectric pipetting system and validate its performance. On the other hand, the system provides the enabling technology in terms of reliability, speed, experiment design flexibility, spot reproducibility and density to make the cancer genomics and functional genomics related research feasible. The ultimate goal of the proposed research is to have the two paths come together, resulting in a fully functional and tested general-purpose, automated, piezoelectric, fluid pipetting system with the reliability and performance to empower cancer related, genomic and functional genomics research.

描述（由申请人提供）：拟议的研究遵循两条不同但互补的路径。一条路径涉及开发和测试新型的压电移液，分配，传感和住房技术，并将其整合到全自动的压电移移移液系统中。创新的基于压电的技术可以使流体和连续监测的亚纳米素体容量移动，以检测堵塞的技巧和其他操作状态。另一条路径涉及与Fred Hutchinson癌症研究中心（F.H.C.R.C.）进行与癌症相关的基因组学研究，以使用拟议的Piezoelectric Postting System进行1）开发更强大的基于寡核苷酸或基于cDNA的微阵列平台的基因表达分析，2）完成开发基于cDNA微阵列的平台，用于同时基因分型和基因表达监测以及3）发展基于细胞功能的新型高通量DNA微阵列测定法，这将使测定沉默特定基因对基因组的表型效应的表型效应是可能的规模。一方面，分子生物学研究提供了一个现实世界的应用，以帮助集中压电移液系统的设计并验证其性能。另一方面，该系统就可靠性，速度，实验设计灵活性，可重现性和密度提供了促成技术，以使癌症基因组学和功能基因组学相关研究可行。拟议的研究的最终目标是将这两条路径融合在一起，从而产生完整的和测试的通用，自动化，压电，流体移液系统，具有可靠性和性能，以赋予与癌症相关的，基因组和功能基因组学研究。