BRUKER ELEXSYS EPR SPECTROMETER

布鲁克 ELEXSYS EPR 光谱仪

• 批准号: 6291972
• 负责人: Jimmy Feix
• 金额: $ 42.1万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2002-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6291972
• 关键词: biomedical equipment purchase; electron spin resonance spectroscopy

The purpose of this proposal is to provide access to a reliable, state-of- the-art X-band CW EPR spectrometer with high sensitivity and time resolution at the Biophysics Research Institute of the Medical College of Wisconsin. Each of these projects employs EPR as the primary analytical tool. The requested instrument will allow the faculty to pursue their NIH-funded projects with a rigor not otherwise possible. Specifically, the projects that will benefit include (1) characterization of ligand-induced conformational changes in the ferric enterobactin receptor as studied by site-directed spin labeling, (2) studies of oxidation/nitration by nitric oxide, (3) reactions between nitric oxide, peroxynitrite, and thiols, (4) analysis of free radical mechanisms of LDL oxidation, (S) examination of endothelial nitric oxide synthase and the radical mechanism of antitumor anthracyclines, (6) EPR studies of the antitumor agent bleomycin and its complexes with DNA, (7) spin label oximetry investigations, (8) studies of the molecular basis of lens transparency, and (9) site-directed spin labeling studies of small heat- shock proteins. Each of these projects has a clear need for increased access to a research-level EPR spectrometer. This proposal seeks funding for an instrument with high sensitivity, accurate temperature control in the range of 4 - 400 K, well-developed and adaptable software, and time resolution on the order of a few milliseconds. EPR is a fundamental technique in studies of protein and metalloprotein structure and in elucidating free radical intermediates in biochemical and pathological processes. Investment in the requested spectrometer will substantially strengthen NIH-supported research.

该提案的目的是在威斯康星医学院生物物理研究所提供可靠、最先进的、具有高灵敏度和时间分辨率的 X 波段 CW EPR 光谱仪。 这些项目均采用 EPR 作为主要分析工具。所要求的工具将使教师能够以其他方式无法实现的严格程度来开展 NIH 资助的项目。具体来说，受益的项目包括（1）通过定点自旋标记研究配体诱导的铁肠杆菌素受体构象变化的表征，（2）一氧化氮氧化/硝化的研究，（3）一氧化氮之间的反应(4) LDL氧化的自由基机制分析，(S)内皮一氧化氮合酶的检查和抗肿瘤蒽环类药物的自由基机制， (6) 抗肿瘤剂博莱霉素及其与 DNA 的复合物的 EPR 研究，(7) 自旋标签血氧测定研究，(8) 晶状体透明度的分子基础研究，以及 (9) 小热源的定点自旋标记研究休克蛋白。这些项目中的每一个都明确需要增加研究级 EPR 光谱仪的使用。该提案寻求资助一种具有高灵敏度、4 - 400 K 范围内精确温度控制、开发良好且适应性强的软件以及几毫秒数量级时间分辨率的仪器。 EPR 是研究蛋白质和金属蛋白质结构以及阐明生化和病理过程中自由基中间体的一项基本技术。对所需光谱仪的投资将大大加强 NIH 支持的研究。