Tissue Specific ECM Scaffold for the Functional Repair of the Vocal Fold Lamina P

用于声带 P 功能修复的组织特异性 ECM 支架

• 批准号: 7645415
• 负责人: Thomas W. Gilbert
• 金额: $ 14.51万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-16 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7645415
• 关键词: 3-Dimensional; Acute; Address; Architecture; Biological; Biomechanics; Bladder; Canis familiaris; Cardiovascular system; Cells; Characteristics; Cicatrix; Clinical; Collagen Fiber; Contralateral; Cues; Data; Development; Disease; Employment; Environment; Epithelial Cells; Evaluation; Extracellular Matrix; Family suidae; Frequencies; Glycosaminoglycans; Goals; Healed; Histologic; Hoarseness; Injury; Intubation; Lamina Propria; Lead; Liver; Malignant Neoplasms; Mechanics; Methodology; Modality; Musculoskeletal System; Natural regeneration; Operative Surgical Procedures; Organ; Otolaryngology; Pathologist; Patients; Phenotype; Physicians; Prevention; Production; Property; Quality of life; Radiation; Regenerative Medicine; Relative (related person); Research; Research Project Grants; Signal Transduction; Site; Small Intestinal Submucosa; Social Interaction; Source; Stem cells; Structure; Structure-Activity Relationship; System; Technology; Testing; Therapeutic; TimeLine; Tissue Engineering; Tissues; Trauma; Voice; Voice Quality; Work; biomechanical engineering; body system; clinical application; clinical practice; design; experience; gastrointestinal system; healing; injured; injury and repair; mechanical behavior; novel; preclinical study; public health relevance; repaired; response; scaffold; success; urologic; vibration; vocal cord; 3-Dimensional; Acute; Address; Architecture; Biological; Biomechanics; Bladder; Canis familiaris; Cardiovascular system; Cells; Characteristics; Cicatrix; Clinical; Collagen Fiber; Contralateral; Cues; Data; Development; Disease; Employment; Environment; Epithelial Cells; Evaluation; Extracellular Matrix; Family suidae; Frequencies; Glycosaminoglycans; Goals; Healed; Histologic; Hoarseness; Injury; Intubation; Lamina Propria; Lead; Liver; Malignant Neoplasms; Mechanics; Methodology; Modality; Musculoskeletal System; Natural regeneration; Operative Surgical Procedures; Organ; Otolaryngology; Pathologist; Patients; Phenotype; Physicians; Prevention; Production; Property; Quality of life; Radiation; Regenerative Medicine; Relative (related person); Research; Research Project Grants; Signal Transduction; Site; Small Intestinal Submucosa; Social Interaction; Source; Stem cells; Structure; Structure-Activity Relationship; System; Technology; Testing; Therapeutic; TimeLine; Tissue Engineering; Tissues; Trauma; Voice; Voice Quality; Work; biomechanical engineering; body system; clinical application; clinical practice; design; experience; gastrointestinal system; healing; injured; injury and repair; mechanical behavior; novel; preclinical study; public health relevance; repaired; response; scaffold; success; urologic; vibration; vocal cord

DESCRIPTION (provided by applicant): Vocal fold injury (due to surgical intervention, cancer, or abuse) leads to the formation of scar tissue that seriously comprises the quality of voice production and can seriously detract from the quality of life for the patient. The current project proposes to investigate an approach for recontruction of vocal fold tissue by utilizing a naturally occurring extracellular matrix scaffold derived from xenogeneic vocal fold tissue. Vocal fold tissue is subjected to extreme magnitudes and frequencies of deformation, and the default mechanism of healing in the vocal fold is the formation of scar tissue which leads to significant clinical problems and quality of life issues. Extracellular matrix (ECM) technology has been shown to be successful for treating a variety of tissue in a clinical setting. The hypothesis for this specific approach is that the specific composition and architecture of the ECM in the vocal fold will provide the most desireable set of biologic signals to guide the development of functionally and morphologically appropriate vocal fold tissue after injury. The scope of this research project will include detailed structural biomechanical analysis to determine how the composition and architecture of the vocal fold allow its highly specific function. Furthermore, one goal of the project is to specifically show how the changes in the tissue composition and architecture after injury lead to alterations in function. This will be accomplished through the development of relevant biomechanical tests that will provide the opportunity for rigorous evaluation of the proposed therapeutic approaches with relevance to the clinical setting. An experienced interdisciplinary team consisting of biomechanical engineers, tissue engineers, physicians, and pathologists has been assembled to conduct these studies. A timeline for completion of these studies and quantitative criteria for success are provided. PUBLIC HEALTH RELEVANCE: Vocal fold scarring is a frequent disorder that can occur as a result of surgical trauma, intubation, vocal abuse, radiation for cancer, and some systemic illnesses. As a result, patients experience reduced quality of voice production (i.e., hoarseness) that can lead to loss of employment, limited social interactions, and generally, a decrease in their quality of life. Treatment modalities must be developed that address the underlying problem of disruption of the native organization and composition of the vocal fold tissue.

描述（由申请人提供）：声带损伤（由于手术干预，癌症或滥用）导致形成疤痕组织，这些疤痕组织严重构成了语音产生的质量，并且可能严重削弱患者的生活质量。当前的项目提议通过利用自然存在的源自异构性声褶皱组织的天然细胞外基质支架来研究声带组织的重建方法。声带组织受到极端幅度和变形的频率，声带中愈合的默认机制是疤痕组织的形成，这会导致重大的临床问题和生活质量问题。细胞外基质（ECM）技术已被证明是在临床环境中成功治疗多种组织的成功。这种特定方法的假设是，ECM在人声折叠中的特定组成和结构将提供最令人期望的生物信号集，以指导受伤后功能和形态上适当的声带组织的发展。该研究项目的范围将包括详细的结构生物力学分析，以确定声带的组成和结构如何允许其高度特异性的功能。此外，该项目的一个目标是专门显示受伤后组织组成和结构的变化如何导致功能改变。这将通过开发相关的生物力学测试来实现，该测试将为拟议的治疗方法进行严格评估，并与临床环境相关。一支由生物力学工程师，组织工程师，医师和病理学家组成的经验丰富的跨学科团队已组成，以进行这些研究。提供了完成这些研究的时间表和成功的定量标准。 公共卫生相关性：声带疤痕是一种常见的疾病，由于手术创伤，插管，声带滥用，癌症的辐射和某些全身性疾病而可能发生。结果，患者的语音产生质量降低（即嘶哑），可能导致就业丧失，社交互动有限，并且通常会降低其生活质量。必须开发治疗方式，以解决本地组织中断和声带组织组成的根本问题。