Core--Laboratory

核心--实验室

• 批准号: 7681792
• 负责人: WALTER E STAMM
• 金额: $ 10.94万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7681792
• 关键词: Antibiotic susceptibility; Base Sequence; Basic Science; Behavioral; Chlamydia; Clinical; Clinical Research; Detection; Ensure; Epidemiologic Studies; Funding; Future; Genomics; Genotype; Gonorrhea; International; Laboratories; Laboratory Research; Molecular Biology; Mycoplasma genitalium; Neisseria gonorrhoeae; Nucleic Acid Amplification Tests; Organism; Other Resources; Polymerase Chain Reaction; Procedures; Pulsed-Field Gel Electrophoresis; Registries; Research Personnel; Serologic tests; Serological; Serotyping; Specimen; Study of serum; Technology; Testing; Trichomonas; Urine; base; cost; experience; major outer membrane protein; success

The STD-TM-CRC laboratory core will 1) provide necessary specialized microbiological and laboratory expertise for investigators performing clinical, epidemiological and behavioral projects within the STD-TMCRC; 2) facilitate the rapid application of new advances in basic sciences or molecular biology to relevant laboratory and epidemiological projects within the STD-TM-CRC; 3) serve as a national and international reference laboratory for other investigators studying STDs; and 4) maintain a chlamydia and gonorrhea registry. The laboratory expertise provided in the core will consist of highly specialized laboratory tests that are not routinely available even in other research laboratories but are integral to the success of proposed epidemiological or clinical research. Examples of such tests include determination of C. trachomatis serovars; determination of gonococcal auxotypes and serotypes; nucleic acid amplification tests for chlamydia, gonorrhea and Mycoplasma genitalium; pulse field gel electrophoresis (PFGE) analysis of N. gonorrhoeae; MOMP nucleotide sequences of selected chlamydia strains; and micro IF serology for C. trachomatis. Provision of these and other tests in a core laboratory ensures that they will be performed by technically experienced investigators, reduces costs by economy of scale, and makes these tests available to researchers conducting behavioral and epidemiological studies who might otherwise not have access to laboratory collaborators. The core will also actively seek to transfer appropriate technology from basic science laboratories to relevant clinical and epidemiological projects. New procedures will be developed and implemented as needed. Examples in this funding period will include nucleic acid amplification tests for trichomonas and M. genitalium and detection of HPV-DNA using urine specimens.The procedures performed within the laboratory core will be available as a national and international resource for other investigators, eliminating the need for them to develop expensive and technically demanding tests.

STD-TM-CRC实验室核心将为1）提供必要的专业微生物和实验室专业知识，为在STD-TMCRC内部执行临床，流行病学和行为项目的研究人员； 2）促进基础科学或分子生物学中新进步的迅速应用于STD-TM-CRC中相关的实验室和流行病学项目； 3）作为研究性病的其他研究人员的国家和国际参考实验室； 4）维持衣原体和淋病注册表。核心提供的实验室专业知识将包括高度专业的实验室测试，这些测试即使在其他研究实验室也无法使用，但对于拟议的流行病学或临床研究的成功是不可或缺的。此类测试的示例包括确定沙眼梭状疱疹血清射手；淋球菌配体和血清型的测定；核酸扩增测试 衣原体，淋病和支原体生殖器；淋病猪笼草的脉冲场凝胶电泳（PFGE）分析；选定衣原体菌株的MOMP核苷酸序列；和微观曲霉的血清学。在核心实验室中提供这些和其他测试可确保他们将由技术经验丰富的研究人员进行，从而降低规模经济，并使这些测试可用于进行行为和流行病学研究的研究人员，否则他们可能无法与实验室合作者访问。核心还将积极寻求将适当的技术从基础科学实验室转移到相关的临床和流行病学项目。新程序将根据需要开发和实施。在此资助期间的例子将包括用于使用尿液标本的滴虫和生殖器的核酸扩增测试以及HPV-DNA的检测。实验室核心内执行的程序将作为其他研究人员作为国家和国际资源提供，从而消除了他们需要开发昂贵和技术要求的测试的需求。