MAPPING OF ALCOHOL PREFERENCE GENES IN RQI STRAINS

RQI 菌株中酒精偏好基因的图谱

• 批准号: 6509244
• 负责人: Csaba Vadasz
• 金额: $ 20.11万
• 依托单位: NATHAN S. KLINE INSTITUTE FOR PSYCH RES
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6509244
• 关键词: alcoholism /alcohol abuse; animal genetic material tag; behavioral /social science research tag; behavioral genetics; gender difference; genetic mapping; genetic markers; genetic polymorphism; genetic strain; genotype; laboratory mouse; preference; psychopharmacology; quantitative trait loci

DESCRIPTION: (Adapted from the Investigator's Abstract) Genetic factors can contribute to the development of alcoholism, a complex human disorder. The long-term goal of this proposal is to identify human genes that predispose to alcohol abuse. Identification of the genetic risk factors offers the potential for prevention and treatment of alcohol abuse. However, identification of human quantitative trait loci (QTLs) for alcoholism has been difficult. The purpose of this work is to continue our studies using animal models of alcoholism, and to focus on the identification and fine mapping of QTLs that affect alcohol preference. For the genetic analysis of complex traits quasi-congenic Recombinant QTL Introgression (RQI) mouse strains were developed in our laboratory. We recently mapped provisional QTLs for ethanol preference to small segments of chromosomes 1, 2, and 15, in a series of RQI strains. Location of these QTLs will be verified and we will search for additional QTLs in a larger series of RQI strains. The specific aims of the present proposal are: (1) to investigate gender differences in preference for 12% (v/v) ethyl alcohol solution by carrying out standard two-bottle-choice experiments with male and female RQI mice, (2) to decrease the current average distance between genetic markers in the quasi-congenic RQI strains by characterizing the strains for 610 microsatellite markers, and to map additional alcohol preference QTLs at 2.5 cM marker spacing in a set of >100 RQI strains, (3) to confirm or reject provisional QTLs in segregating generations, and (4) to reduce the length of chromosome segments that harbor confirmed QTLs to less than 1 cM intervals by utilizing individuals that carry recombinant chromosome segments in segregating populations. Identification of a <1 cM region will allow the identification of candidate genes and ESTs. We will map each identified and confirmed QTL.

描述：（根据研究者的摘要进行了改编）遗传因素可以 有助于酗酒的发展，这是一种复杂的人类障碍。这 该提案的长期目标是确定易感的人类基因 酗酒。遗传危险因素的识别提供了潜力 用于预防和治疗酒精滥用。但是，人类的识别 酗酒的定量性状基因座（QTL）很困难。目的 这项工作是要使用酒精中毒模型继续我们的研究， 专注于影响酒精的QTL的识别和精细映射 偏爱。用于复杂性状的遗传分析准综合 重组QTL渗入（RQI）小鼠菌株在我们的 实验室。我们最近绘制了用于乙醇偏爱小的临时QTL 一系列RQI菌株中染色体1、2和15的片段。位置 这些QTL将得到验证，我们将在较大的 系列RQI菌株。本提案的具体目的是：（1） 研究12％（v/v）乙醇的性别差异 通过对男性进行标准的两瓶选择实验，并 雌性RQI小鼠，（2）降低遗传之间的当前平均距离 通过表征610的菌株，准综合RQI菌株中的标记物 微卫星标记，并在2.5 cm处绘制额外的酒精偏好QTL 标记间距在一组> 100个RQI菌株中，（3）确认或拒绝 分离世代的临时QTL，（4）减少长度 染色体片段携带确认QTL少于1 cm 利用携带重组染色体段的个体在分离中 人群。 <1 cm区域的识别将允许识别 候选基因和EST。我们将绘制每个已识别并确认的QTL。

项目成果

mGluR7 genetics and alcohol: intersection yields clues for addiction.

• DOI: 10.1007/s11064-011-0452-z
• 发表时间: 2011-06
• 期刊: Neurochemical research
• 影响因子: 4.4
• 作者: Gyetvai B;Simonyi A;Oros M;Saito M;Smiley J;Vadász C
• 通讯作者: Vadász C

Glutamate receptor metabotropic 7 is cis-regulated in the mouse brain and modulates alcohol drinking.

• DOI: 10.1016/j.ygeno.2007.08.006
• 发表时间: 2007-12
• 期刊: Genomics
• 影响因子: 4.4
• 作者: C. Vadász;M. Saito;B. Gyetvai;M. Oros;I. Szakall;Krisztina M. Kovacs;V. Prasad;R. Toth

Mouse striatal transcriptome analysis: effects of oral self-administration of alcohol.

小鼠纹状体转录组分析：口服自我饮酒的影响。

• DOI: 10.1016/j.alcohol.2004.02.005
• 发表时间: 2004
• 期刊: Alcohol (Fayetteville, N.Y.)
• 作者: Saito,Mariko;Szakall,Istvan;Toth,Reka;Kovacs,KrisztinaM;Oros,Melinda;Prasad,VidudalaVTS;Blumenberg,Miroslav;Vadasz,Csaba
• 通讯作者: Vadasz,Csaba

G9a-mediated histone methylation regulates ethanol-induced neurodegeneration in the neonatal mouse brain.

• DOI: 10.1016/j.nbd.2013.01.022
• 发表时间: 2013-06
• 期刊: Neurobiology of disease
• 影响因子: 6.1
• 作者: Subbanna S;Shivakumar M;Umapathy NS;Saito M;Mohan PS;Kumar A;Nixon RA;Verin AD;Psychoyos D;Basavarajappa BS
• 通讯作者: Basavarajappa BS

Scanning of five chromosomes for alcohol consumption loci.

扫描五条染色体的饮酒位点。

• DOI: 10.1016/s0741-8329(00)00098-7
• 发表时间: 2000
• 期刊: Alcohol (Fayetteville, N.Y.)
• 作者: Vadasz,C;Saito,M;Gyetvai,B;Mikics,E;Vadasz2nd,C
• 通讯作者: Vadasz2nd,C