INIA: Genotyping Core

INIA：基因分型核心

• 批准号: 6622588
• 负责人: ROBERT W WILLLIAMS
• 金额: $ 14.3万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6622588
• 关键词: alcoholism /alcohol abuse; animal genetic material tag; animal tissue; behavioral /social science research tag; behavioral genetics; biomedical facility; cooperative study; gel electrophoresis; gene mutation; genetic mapping; genetic markers; genetic polymorphism; genome; genotype; high throughput technology; informatics; information dissemination; nucleic acid sequence; polymerase chain reaction; quantitative trait loci

DESCRIPTION (provided by applicant): Alcoholism is a complex multifactorial problem that is caused in part by genetic differences. In common with other components of the MA, this core is contributing the analysis of the molecular substrates of alcohol consumption by providing the INIA and NIAAA-supported investigators with free access to a high-throughput genotyping core facility and associated bioinformatic resources. The Genotyping Core provides gene mapping and sequencing services to the four major research components and to pilot and exploratory INIA components. The Core provides high resolution mapping with fast turn-around. Unlike other genotyping facilities, this Core provides complete service-from DNA extraction through to genetic maps. The Core has markers for over 2000 microsatellite that are highly polymorphic and suitable for analysis of experimental crosses. The Core has several major service related aims: 1. To provide genotyping of progeny of crosses between knockout carriers. 2. To map novel mutations and QTLs generated by INIA Research Component. This service involves both the analysis of alcohol-consumption mutants generated by the Tennessee Mouse Genome Consortium and by the INIA?s own recessive ENU screen. 3. To assist the genetic tracking and generation of consomic and congenic lines. 4. To provide high resolution genetic maps for new recombinant and advanced recombinant inbred strains generated by several research groups. 5. To provide genotyping services to pilot and exploratory INIA applications. 6. To provide other NIAAA-funded investigators technical expertise and genotypes for selected projects. The INIA genotyping core will make significant contributions to the analysis of possible genetic determinants of alcohol consumption in mice and to the analysis of the genetic basis of the correlation between high stress and high alcohol consumption in human populations. Our goal is efficient service to INIA and NIAAA investigators.

描述（由申请人提供）： 酒精中毒是一个复杂的多因素问题，部分原因是 遗传差异。与MA的其他组件共同，此核心是 促进对饮酒的分子底物的分析 通过为INIA和NIAAA支持的调查人员提供免费访问 高通量基因分型核心设施和相关的生物信息 资源。基因分型核心提供基因映射和测序服务 到四个主要的研究组件，并进行试点和探索性Inia 成分。核心提供了高分辨率映射，并通过快速的转弯。 与其他基因分型设施不同，该核心提供完整的服务。 DNA提取到遗传图。核心有2000年以上的标记 高度多态性且适合分析的微卫星 实验十字架。核心有几个主要服务的目的：1。 提供敲除载体之间十字架后代的基因分型。 2。映射 INIA研究成分产生的新型突变和QTL。这项服务 既涉及对酒精消费突变体的分析 田纳西州小鼠基因组联盟和Inia自己的隐性ENU屏幕。 3。协助遗传跟踪和生成综合和先天 线。 4。为新的重组和高分辨率的遗传图和 几个研究小组产生的晚期重组近交菌株。 5。 为试点和探索性INIA应用提供基因分型服务。 6。 为了提供其他NIAAA资助的研究人员的技术专长和基因型 适用于选定的项目。 INIA基因分型核心将使 分析酒精的遗传决定因素的贡献 小鼠的消耗和分析的遗传基础 人类高压力与高酒精消耗之间的相关性 人群。我们的目标是为INIA和NIAAA调查人员提供有效的服务。