CORE--TRANSGENIC AND GENE TARGETED MOUSE

核心——转基因及基因靶向小鼠

• 批准号: 6651375
• 负责人: Ju Chen
• 金额: $ 10.66万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-25 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6651375
• 关键词: animal colony; biomedical facility; gene targeting; genetically modified animals; laboratory mouse

DESCRIPTION (provided by applicant): The three projects in the Program will utilize a combination of transgenic and gene-targeted approaches to study the role of candidate genes with the potential important functional roles in cardiac function, which are identified in these projects. Core Unit B will provide services to assist in the design of the appropriate constructs and the subsequent generation of gene-targeted and transgenic mice. The objectives are five-fold: 1) to design and test appropriate transgenes in in vitro systems; 2) to generate appropriate lines of transgenic and gene-targeted mice for various projects in the Program; 3) to identify founders and to breed/maintain appropriate lines of mice; 4) to knockout specific candidate regulatory genes via homologous recombination; and 5) to breed and generate lines of mice required for regionally restricted and/or tissuespecific conditional gene targeting and over-expression via CRE-lox strategies.

描述（由申请人提供）： 该计划中的三个项目将结合转基因和基因靶向技术 研究具有潜在重要功能作用的候选基因的作用的方法 在这些项目中确定了心脏功能。核心单元 B 将提供服务，协助设计适当的构建体以及随后生成基因靶向和转基因小鼠。目标有五个：1）在体外系统中设计和测试适当的转基因； 2) 为该计划的各个项目培育合适的转基因和基因靶向小鼠品系； 3) 确定创始人并培育/维持适当的小鼠品系； 4）通过同源重组敲除特定的候选调控基因； 5)通过CRE-lox策略培育和产生区域限制和/或组织特异性条件基因靶向和过度表达所需的小鼠品系。