Transgenic/Knockout Mice

转基因/基因敲除小鼠

• 批准号: 7081710
• 负责人: ASTAR WINOTO
• 金额: $ 23.68万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-15 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7081710
• 关键词: T cell receptor; animal care; animal colony; biomedical facility; biotechnology; cellular immunity; disease /disorder model; gene targeting; genetic polymorphism; genetic promoter element; genetic strain; genetically modified animals; green fluorescent proteins; host organism interaction; immunogenetics; immunoregulation; laboratory mouse; major histocompatibility complex; microorganism immunology; parasite infection mechanism; virus infection mechanism

The ability to manipulate the mouse genome through transgenic and knock-out or knock-in technologies has revolutionized modern Immunology. The purpose of this core facility (Core B) is to integrate the transgenic and gene targeting approach into the studies of host-pathogen interaction, a major thrust of the program project "Mouse models for understanding host-pathogen interactions". This Transgenic/Knockout Mouse Core will provide the critical technical capability to generate and to maintain transgenic and mutant mice commonly used by two or more investigators of the program project (PPG). In particular, this Core will complement Core C to provide transgenic mice expressing fluorescent proteins to be used for imaging studies. Core B will also generate and distribute transgenic strains of mice as needed by the individual investigator in the PPG. In aim 1, C57Bl/6 transgenic mice expressing fluorescent genes, whose expression is driven by different promoter/enhancers will be generated and maintained for use in video imaging experiments. These include mice expressing membrane Cyan Fluorescent Protein (CFP) in dendritic cells or macrophages and mice with CFP or YFP (Yellow Fluorescent Protein) expressed in all cell types. In aim 2, knock-out/knock-in mice with genes involved in innate immunity will be maintained as well as back-crossed to C57BI/6 or Balb/c if necessary. These mice, including DC- and macrophage-specific FADD-/-, MyD88-/-, IFN-alpha/betaR-/-, TRAIL-R-/-, NK deficient mice, NKG2D-/-, IL-12/GFP (Green Fluorescent Protein) knock-in and lFN-gamma/GFP knock-in mice, will be provided to investigators in the PPG for their biochemical, infection or imaging experiments. In aim 3, Core B will generate and provide transgenic mice for the specific needs of the individual PPG investigator. Completion of these aims is necessary and essential for the success of the PPG designed to understand how the immune system interacts with model pathogens.

通过转基因和敲除或敲除技术操纵小鼠基因组的能力 已经彻底改变了现代免疫学。该核心设施的目的（核心B）是整合 转基因和基因靶向宿主 - 病原体相互作用的方法，这是该研究的主要力量 程序项目“用于理解宿主病原体相互作用的小鼠模型”。这 转基因/敲除鼠标核心将提供关键的技术能力，以生成和维护 该计划项目（PPG）的两个或多个研究者通常使用的转基因和突变小鼠。 特别是，该核心将补充Core C以提供表达荧光蛋白的转基因小鼠 用于成像研究。核心B还将生成和分布小鼠的转基因菌株 PPG中的个别研究人员需要。在AIM 1中，C57BL/6表达的转基因小鼠 荧光基因的表达是由不同的启动子/增强子驱动的，并且会产生 维护用于视频成像实验。这些包括表达膜青色的小鼠 树突状细胞或巨噬细胞中的荧光蛋白（CFP）以及CFP或YFP的小鼠（黄色 荧光蛋白）在所有细胞类型中表达。在AIM 2中，与涉及基因的敲除/敲门小鼠 如果需要，将保持先天免疫力，并在必要时进行后跨至C57BI/6或BALB/C。这些 小鼠，包括DC-和巨噬细胞特异性FADD - / - ，MyD88 - / - ，IFN-Alpha/betar - / - ，trail-r-/ - ，NK不足 小鼠，NKG2D - / - ，IL-12/GFP（绿色荧光蛋白）敲入和LFN-gamma/gfp敲击小鼠，将是 提供给PPG的研究人员的生化，感染或成像实验。在AIM 3中， 核心B将生成并提供转基因小鼠，以满足单个PPG的特定需求 研究者。这些目标的完成对于旨在成功的PPG的成功至关重要 了解免疫系统如何与模型病原体相互作用。