BREAST CANCER HYPOXIA ASSESSED BY NITRIC OXIDE

通过一氧化氮评估乳腺癌缺氧

• 批准号: 6514636
• 负责人: BRITTON CHANCE
• 金额: $ 10.34万
• 依托单位: MEDICAL DIAGNOSTIC RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-16 至 2004-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6514636
• 关键词: breast neoplasms; clinical research; hemoglobin; human subject; hypoxia; infrared spectrometry; metastasis; nitric oxide; polarography

DESCRIPTION: (Applicant's Description) It is our intent here to lay the groundwork for a translational study of the efficacy of NIR optical method in deternining the level of hypoxia in human breast cancer by NIR optical spectroscopy and imaging. The test requires a gold standard of breast tumor hypoxia which is to be provided by pO2 deterninations where the instrumentation, expertise and technology is available at the Leipzig University while the expertise and technology for NIR spectroscopy and imaging is available at the University of Pennsylvania affording an opportunity for translational research. It is our intent to mobilize activities at these two locations in order that a initial test of 50 breast cancers can be made to validate that the spectroscopy and imaging of breast tumor hypoxia gives values comparable to those obtained with the gold standard of pO2 determination and that the tumor localization is consistent with other modalities particularly ultrasound, mammography and biopsy. Thus these studies require validation that these two groups can work together; the Philadelphia group providing instrumentation and personnel skilled in use of the instrumentation, whilst the Leipzig group will provide not only expertise in pO2 determination, ultrasound, biopsy, marnnography, but also the capability of making available a large population of high risk breast cancer patients who are willing to accept the complete protocol for breast cancer examinations. It is our intent to provide two types of NIR information: I) localized and quantitative spectroscopy of the desaturation of hemoglobin and the blood content in the same breast canceri region as that in which the pO2 deternination takes place. 2) A NIR imaging system which gives rapid and effective imaging of the deoxygenation of hemoglobin and blood concentration relative to nearby normal breast tissue. While the pO2 determinations and NIR hemoglobin spectroscopy and imaging currently afford the basis for calibration in models, the comparative study has never been done before in human breast cancers. If ourintended goals of demonstrating feasibility are achieved, then application for support through regular RO1channels would appear to be justified.

描述：（申请人的描述） 我们在这里打算为翻译研究奠定基础 NIR光学方法在确定人类缺氧水平的功效 NIR光谱和成像通过乳腺癌。测试需要一个 PO2提供的乳腺肿瘤缺氧的黄金标准 仪器，专业知识和技术在哪里 可在莱比锡大学获得，同时又有NIR的专业知识和技术 宾夕法尼亚大学可以使用光谱和成像 为翻译研究提供了机会。这是我们的意图 动员在这两个地点的活动，以便初始测试为50 可以进行乳腺癌来验证光谱和成像 乳腺肿瘤缺氧的值与金获得的值相当 PO2确定的标准和 肿瘤定位与其他方式一致 超声检查，乳房X线摄影和活检。因此，这些研究需要验证 这两个小组可以一起工作。费城集团提供 仪器和人员熟练使用仪器，同时 莱比锡集团不仅将提供PO2确定方面的专业知识，还将提供专业知识， 超声检查，活检，慈善事业，但也可以提供可用的能力 大量的高风险乳腺癌患者愿意 接受完整的乳腺癌检查方案。这是我们的意图 提供两种类型的NIR信息：i）本地化和定量 血红蛋白的去饱和度和血液含量的光谱法 与PO2恶化的乳腺癌区域相同。 2）一个NIR成像系统，可快速有效地成像 相对于附近正常的血红蛋白和血液浓度的脱氧 乳房组织。而PO2测定和NIR血红蛋白光谱法 成像目前为模型中的校准提供了基础 比较研究从未在人类乳腺癌中进行过。如果 我们实现了证明可行性的目标，然后应用 通过常规的RO1通道进行支持，似乎是合理的。