Nanoscale Devices on DNA Functionalized Semiconductors

DNA 功能化半导体纳米级器件

• 批准号: 6585519
• 负责人: DAVID S GINGER
• 金额: $ 3.42万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-07-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6585519
• 关键词: DNA; atomic force microscopy; biosensor device; electron transport; fluorescence resonance energy transfer; microarray technology; nanotechnology; nucleic acid structure; oligonucleotides; printing; technology /technique development

DESCRIPTION (provided by applicant): The goal of this research program is to interface semiconductor circuitry with nanoparticle-based DNA detection schemes using the technique of Dip Pen Nanolithography (DPN). The project will develop optimize the chemistry needed to immobilize oligonucleotides on semiconductor surfaces in a manner compatible with the DPN process. Modifications of the atomic force microscope/DPN tip to enhance the efficiency of oligonucleotide ink deposition will be pursued and the DPN fabricated substrates will be used to assemble and characterize patterns of DNA functionalized nanoparticles. The project will develop and study new single-label detection schemes based on the electrical and near-field-optical properties of nanoparticles with surface-bound DNA, and will develop proof-of-principle demonstrations of integrated nanoscale biosensor circuitry. Not only do these schemes offer the promise of enhanced sensitivity and selectivity as compared to fluorophore-based detection methods, but their development also addresses fundamental issues of nanoscience in biological systems including those of diffusion, charge transport and energy transfer.

描述（由申请人提供）：该研究计划的目标是 将半导体电路与基于纳米颗粒的 DNA 检测方案连接起来 使用浸笔纳米光刻（DPN）技术。该项目将发展 优化将寡核苷酸固定在半导体上所需的化学反应 表面以与 DPN 过程兼容的方式。的修改 原子力显微镜/DPN 尖端可提高寡核苷酸的效率 将采用油墨沉积并使用 DPN 制造的基板 组装和表征 DNA 功能化纳米粒子的模式。这 项目将开发和研究新的单标签检测方案 纳米粒子的电学和近场光学特性 表面结合 DNA，并将开发原理验证演示 集成纳米级生物传感器电路。这些计划不仅提供 与相比，有望提高灵敏度和选择性 基于荧光团的检测方法，但它们的发展也解决了 生物系统中纳米科学的基本问题，包括 扩散、电荷传输和能量转移。

项目成果

(E)-2-[(2-Hydr-oxy-5-nitro-phen-yl)iminiometh-yl]-4-nitro-phenolate.

(E)-2-[(2-羟基-5-硝基-苯基)亚氨基甲基]-4-硝基-苯酚盐。

• DOI: 10.1107/s1600536809000543
• 发表时间: 2009
• 期刊: Acta crystallographica. Section E, Structure reports online
• 作者: Hijji,YousefM;Barare,Belygona;Butcher,RayJ;Jasinski,JerryP
• 通讯作者: Jasinski,JerryP