CHARACTERIZATION AND QUANTIFICATION OF IMMOBILIZED DNA

固定化 DNA 的表征和定量

基本信息

批准号：
6344122
负责人：
FEIMENG ZHOU
金额：
$ 7.32万
依托单位：
CALIFORNIA STATE UNIVERSITY LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
1978
资助国家：
美国
起止时间：
1978-06-14 至 2004-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6344122
关键词：
DNA atomic force microscopy biosensor device electrochemistry mass spectrometry membrane reconstitution /synthesis metal complex molecular assembly /self assembly molecular film nucleic acid probes nucleic acid quantitation /detection oligonucleotides polynucleotides scanning electron microscopy

项目摘要

In the proposed work, various schemes for fabricating well-ordered oligonucleotide and polynucleotide films onto gold surfaces will be explored. Emphasis will be placed on the formation of self-assembled monolayers (SAMs) containing DNA molecules or attaching DNA molecules onto preformed SAMs of alkanethiols. These films will be quantified and characterized using a variety of surface and analytical techniques. Five techniques will be employed in an effort to provide a detailed description about the heterogeneous DNA sensor surface: quartz crystal microbalance, atomic force microscope, scanning electrochemical microscope, voltammetry, and electrochemical inductively coupled plasma (ICP)-atomic emission spectrometry ICP-mass spectrometry. These techniques complement one another in terms of studies of DNA immobilization, hybridization intercalation, and film desorption. We intend to integrate the design of the DNA immobilization scheme, the characterization of the sensing surface, and the analytical performance of the resulting sensor of the resulting sensor. Our ultimate objective is to understand certain important factors governing DNA sensor performance (e.g., surface coverage of immobilized DNA, DNA molecular orientation, optimal concentrations of the probe and target for an analysis, and the development of an amenable technique for sensitive and selective detection of nucleic acids) at molecular levels.

在拟议的工作中，将探索各种用于制造有序的寡核苷酸和多核苷酸膜到金表面上的方案。重点将放在含有DNA分子的自组装单层（SAM）或将DNA分子附着在预先形成的烷硫醇的Sams上的形成。这些薄膜将使用各种表面和分析技术进行量化和表征。 Five techniques will be employed in an effort to provide a detailed description about the heterogeneous DNA sensor surface: quartz crystal microbalance, atomic force microscope, scanning electrochemical microscope, voltammetry, and electrochemical inductively coupled plasma (ICP)-atomic emission spectrometry ICP-mass spectrometry.这些技术在DNA固定，杂交插入和膜解吸方面相互补充。我们打算整合DNA固定方案的设计，传感表面的表征以及所得传感器所得传感器的分析性能。我们的最终目标是了解有关DNA传感器性能的某些重要因素（例如，固定的DNA的表面覆盖，DNA分子取向，探针的最佳浓度和分析靶标的最佳浓度以及分析敏感和选择性检测核酸的敏感技术）在分子水平上。