ROLE OF SLO GENES & ION CHANNELS IN NEURONAL DEVELOPMENT

SLO 基因的作用

• 批准号: 6540798
• 负责人: Angeles Badell Ribera
• 金额: $ 4.03万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6540798
• 关键词: South America; Xenopus oocyte; biophysics; calcium channel; complementary DNA; cooperative study; developmental genetics; developmental neurobiology; electrophysiology; embryo /fetus tissue /cell culture; gene expression; membrane channels; messenger RNA; molecular biology; neurons; polymerase chain reaction; potassium channel; protein structure function; synaptogenesis

This application is for support of collaborative research conducted by a U.S. Principal Investigator (Angeles B. Ribera) and a Foreign Scientist (Manuel Kukuljan; Chile). The principal goals of the proposed research are to isolate cDNAs corresponding to the Xenopus orthologue of Slo (xSlo). The Slo gene encodes the pore-forming "-subunit of large conductance potassium (BK) channels. In Xenopus embryonic spinal neurons, BK channels undergo a developmentally regulated change in their sensitivity to calcium. By isolating variants of Slo expressed in embryonic spinal neurons during the developmental period of interest, the investigators will examine the possibility that alternatively spliced variants of xSlo are expressed and that these xSlo variants determine the observed developmentally regulated calcium sensitivity of BK channels. There are 3 Specific Aims: 1) isolate the Xenopus laevis orthologue of the Slo gene (xSlo), 2) analyze expression of xSlo and alternatively spliced forms in Xenopus embryonic spinal neurons, and 3) determine the functional properties of xSlo and variants. The proposed experiments combine embryological, molecular and electrophysiological techniques. The majority of the experiments will be carried out at the foreign site. The long-term goal of this research is to obtain molecular entry into developmental pathways that regulate ion channel expression and function.

该申请是为了支持美国进行的协作研究 首席研究员（Angeles B. Ribera）和外国科学家（Manuel kukuljan;智利）。拟议研究的主要目标是隔离 对应于SLO（XSLO）的爪蟾直系同源物的cDNA。 SLO基因 编码大电导钾（BK）的孔形成孔。 频道。在Xenopus Embryonic脊柱神经元中，BK通道经历A 发育调节其对钙的敏感性的变化。通过隔离 发育过程中在胚胎脊柱神经元中表达的SLO的变体 感兴趣的时期，调查人员将研究 或者表达XSLO的剪接变体，这些XSLO 变体确定观察到的发育调节的钙敏感性 BK频道。有3个具体目的：1）隔离爪蟾laevis SLO基因的直系同源物（XSLO），2）分析XSLO和 替代地剪接胚胎脊柱神经元中的剪接形式，3） 确定XSLO和变体的功能特性。提议 实验结合了胚胎，分子和电生理学 技术。大多数实验将在外国进行 地点。这项研究的长期目标是获得分子进入 调节离子通道表达和功能的发育途径。