Role of Arrestin in Olfactory Desensitization

Arrestin 在嗅觉脱敏中的作用

• 批准号: 6487803
• 负责人: Elaine M Smith
• 金额: $ 2.27万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6487803
• 关键词: Anopheles; Drosophilidae; G protein; arrestins; arthropod genetics; behavior test; behavioral /social science research tag; chemoreceptors; communicable disease transmission; electrophysiology; ethology; gene expression; gene mutation; genetically modified animals; host organism interaction; immunocytochemistry; malaria; molecular psychobiology; olfactions; polymerase chain reaction; protein structure function; receptor coupling; receptor sensitivity; sensory discrimination; species difference; western blottings

DESCRIPTION (provided by applicant): The aim of this research is to contribute to the understanding of olfaction in the malaria vector mosquito Anopheles gambiae, especially as it pertains to host choice and vectorial capacity. Malaria continues to be a serious threat and demands focused investigation. The experiments outlined in this proposal seek to characterize one of the molecular components of olfaction in A. gambiae, an arrestin gene, AgArr1. As arrestins are known to desensitize heptahelical receptors (such as odorant receptors), these studies will initially investigate the role of arrestin genes in olfactory pathways using established mutants in the well-characterized Drosophila melanogaster model system. Behavioral and physiological paradigms including odor-baited traps and electroantennogram (EAG) recordings will be employed to identify and define olfactory phenotypes associated with the loss of insect arrestins. Subsequently, transgenic expression of AgArr1 in Drosophila will be performed to test the ability of the mosquito gene to compensate for these mutations and rescue the phenotype. In order to answer whether AgArr1 plays a similar role in mosquito olfactory pathways, double-stranded RNA interference gene silencing techniques will be utilized to decrease AgArr1 expression in A. gambiae for phenotypic analysis by EAG. These experiments will not only define olfactory deficits associated with loss of arrestin expression in insects, but also examine potential targets for antimalarial vector control.

描述（由申请人提供）：这项研究的目的是贡献 了解疟疾媒介蚊子按蚊的嗅觉 冈比亚，特别是与宿主选择和媒介能力有关。 疟疾仍然是一个严重威胁，需要重点调查。这 该提案中概述的实验旨在表征分子之一 冈比亚曲霉嗅觉成分，抑制蛋白基因 AgArr1。作为抑制蛋白 已知可以使七螺旋受体（例如气味受体）脱敏， 这些研究将首先调查视紫红质抑制蛋白基因在嗅觉中的作用 使用已充分表征的果蝇中已建立的突变体的途径 黑腹果蝇模型系统。行为和生理范式包括 将采用气味诱饵陷阱和触角电图（EAG）记录来 识别和定义与昆虫损失相关的嗅觉表型 抑制蛋白。随后，AgArr1在果蝇中的转基因表达将被 进行测试蚊子基因补偿这些的能力 突变并挽救表型。为了回答 AgArr1 是否扮演 双链RNA干扰在蚊子嗅觉通路中的相似作用 基因沉默技术将用于降低 A. 中的 AgArr1 表达。 冈比亚通过 EAG 进行表型分析。这些实验不仅将定义 昆虫嗅觉缺陷与抑制蛋白表达丧失相关，但是 还研究抗疟媒介控制的潜在目标。