CORE--PEPTIDE BIOCHEMISTRY

核心--肽生物化学

• 批准号: 6564260
• 负责人: JOSEPH R REEVE
• 金额: $ 14.67万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564260
• 关键词: biochemistry; biomedical facility; capillary electrophoresis; fluorescent dye /probe; gastrointestinal system; high performance liquid chromatography; nucleic acid probes; peptide chemical synthesis; protein sequence; radioimmunoassay; synthetic peptide

DESCRIPTION (Adapted from the application) The main objectives of this core are to provide a number of specialized peptides and molecular probes in a cost-effective manner. Many studies performed at CURE utilize peptide reagents. core investigators are trained in how to make, store, dilute and evaluate peptides properly under experimental conditions. The core helps in characterization of natural or synthetic peptides by amino acid analysis, high performance capillary electrophoresis, high performance liquid chromatography (HPLC), mass spectral analysis and microsequence analysis. The core also provides training, reagents and equipment facilities for the radioimmunoassay as well. Investigators are also provided standard or heavily used peptides either in their native form or modified to impart features such as fluorescence or labeling sites. Molecular probes such as oligodeoxyribonucleotides or cDNA probes are provided and analyzed for appropriate quality to ensure that the acquired or custom made probes are of the highest quality. The core is housed in three different laboratories: The Peptide Biochemistry Laboratory (VA); The Nucleic Acid Probes Laboratory (VA) and the Peptide Synthesis and Purification Laboratory (UCLA). The core laboratories are well equipped with all the basic equipment, such as HPLC, dual wavelength UV detector, variable wavelength UV detector, gamma counter, a variety of HPLC columns and centrifuges. Other specialized techniques, such as microsequence, NMR, circular dichroism, mass spectrometry measurements, are performed at various other UCLA laboratories coordinated by the core. The core facilities are currently being utilized by 25 CURE investigators in their projects, and the utilization had resulted in 87 publications during the previous funding period.

描述（根据应用程序改编）该核心的主要目标是 在A中提供许多专门的肽和分子探针 具有成本效益的方式。在治疗上进行的许多研究都利用肽试剂。 核心调查人员接受了如何制作，存储，稀释和评估的培训 在实验条件下正确的肽。核心有助于 通过氨基酸分析对天然或合成肽的表征，高 性能毛细管电泳，高性能液相色谱 （HPLC），质谱分析和微序列分析。也是核心 为放射免疫分子提供培训，试剂和设备设施 也是如此。还提供了标准或大量使用的肽 以其天然形式或修改以赋予诸如荧光之类的特征 或标记网站。分子探针，例如寡脱氧核糖核苷酸或cDNA 提供并分析了适当质量的探针，以确保 获得或定制的探针是最高质量的。核心被安置 在三个不同的实验室中：肽生物化学实验室（VA）；这 核酸探针实验室（VA）和肽合成和纯化 实验室（UCLA）。核心实验室配备了所有基本 设备，例如HPLC，双波长紫外检测器，可变波长紫外线 检测器，伽马计数器，各种HPLC柱和离心机。其他 专业技术，例如微序列，NMR，圆形二色性，质量 光谱测量值是在其他各种UCLA实验室进行的 由核心协调。核心设施目前正在使用25 治愈其项目中的调查人员，并且利用率已导致87 出版物在上一个资金期间。