CORE--NMR

核磁共振

• 批准号: 6576204
• 负责人: Michael P Stone
• 金额: $ 17.41万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6576204
• 关键词: biomedical equipment resource; biomedical facility; environmental toxicology; nuclear magnetic resonance spectroscopy

The NMR Core Facility provides the Center and other units on campus with state-of-the-art NMR spectroscopy. The NMR facility, presently located in the Chemistry Department is operated on an "open shop" basis. Dr. Michael Stone is the leader of the Core and he is assisted by Mr. Markus Voehler who operates, troubleshoots and maintains the equipment and trains new users. The services of Mr. Stan Bottoms, a staff electronics specialist are also utilized. Center investigators, Drs. Harris who has had an influential role in the operation of the facility provides consultation to Center personnel. He and Dr. Marnett are major collaborators with Dr. Stone. Routine analyses as well as advanced multi-dimensional NMR techniques for complex structures are performed. There are presently 200MHz, 300MHz, 400MHz and 500MHz instruments within the facility. The 500MHz NMR has been upgraded to include 3RF channels and field gradient capabilities, and console upgrades are underway for the 300MHz and 400MHz instruments. A total of six Silicon Graphics workstations are used to work up spectra and conduct molecular modeling and dynamics studies. A new partnership has been created between the Vanderbilt University Medical Center, Vanderbilt University Central which includes the College of Arts and Science, the Peabody College and the School of Engineering to operate a newly designated Vanderbilt University Magnetic Resonance Center (VUMRC). In addition to the existing equipment, a 600MHz and an 800MHz NMR as well as a 3 Tesla whole body imaging system will be added to this Center. Additional technical support to assist the current NMR specialist, Markus Voehler will be appointed. It is anticipated that Dr. Stone and Mr. Voehler will be involved in the management of the new University-wide VUMRC. During the present funding period, Center investigators constituted the majority of utilization of the NMR Core Facility. By far the heaviest users of the facility were members of the DNA Damage and Mutagenesis Research Core. The facility, however, also serviced the Enzymatic Oxidation and Conjugation Research Core, the Oxidative Damage Research Core, and the Environmental Pathology Research Core. Ninety-two peer-reviewed publications reported results obtained from the NMR facility. A plan exists for prioritizing the usage of the NMR Core Facility. There are two types of users; those who require routine NMR services for structure proof, and those who use the full range of 2-D capabilities for structural biology work. The work for the former is done largely with the 300 MHZ NMR, while the other three instruments are dedicated for 2-D and other advanced techniques on a scheduled basis. The 600 and 800 MHZ instruments when they arrive will also be used for the latter purposes. Access to the instruments in the Core Facility are through "hands on" training by Markus Voehler or via collaboration with Center investigators or Core personnel. In the next grant period heavier use of the NMR Facility is expected from the Environmental Pathology and Enzymatic Oxidation and Conjugation Research Cores. The Center contributes 75% of the hourly use charges accrued by Center personnel, less is contributed by their academic units. The Center also provides a portion (15%) of Dr. Stone?s salary. The Center has also contributed considerably to the purchase of new equipment. Overall, the Center contribution to the laboratory operating expenses is directly in proportion of its utilization of the equipment.

NMR核心设施为中心和其他单位提供 具有最先进的NMR光谱的校园。 NMR设施，目前 位于化学部门以“开放式商店”的基础运营。 迈克尔·斯通（Michael Stone）是核心的领导者，马克斯先生的协助 操作，进行故障排除和维护设备并训练新的Voehler 用户。员工电子专家Stan Bottoms先生的服务是 也利用。中心调查人员，博士。哈里斯有影响力 该设施运营中的作用为中心提供咨询 人员。他和Marnett博士是Stone博士的主要合作者。 常规分析以及高级多维NMR技术 进行复杂的结构。目前有200MHz，300MHz，400MHz 设施中的500MHz仪器。 500MHz NMR已升级 包括3RF频道和现场梯度功能以及控制台升级 300MHz和400MHz仪器正在进行中。总共六硅 图形工作站用于工作光谱并进行分子 建模和动力学研究。在 范德比尔特大学医学中心，范德比尔特大学中央 包括艺术与科学学院，皮博迪学院和学校 工程运营新指定的范德比尔特大学磁性 共振中心（VUMRC）。除现有设备外，600MHz 800MHz NMR以及3 Tesla全身成像系统将是 添加到这个中心。额外的技术支持以帮助当前 NMR专家Markus Voehler将被任命。预计 Stone博士和Voehler先生将参与新的管理 大学范围的VUMRC。在目前的资金期间，中心 研究人员构成了NMR核心的大部分利用 设施。到目前为止，该设施中最重的用户是DNA的成员 损害和诱变研究核心。但是，该设施也维修了 酶促氧化和结合研究核心，氧化损伤 研究核心和环境病理研究核心。九十二 同行评审的出版物报告了从NMR设施获得的结果。 存在一个计划，以优先使用NMR核心设施。有 两种用户；那些需要常规的NMR服务的人 证明和那些使用完整二维功能进行结构的人 生物学工作。前者的工作很大程度上是在300 MHz NMR的情况下完成的 而其他三种乐器专用于2-D和其他高级 预定的技术。 600和800 MHz仪器 到达也将用于后一个目的。访问乐器 在核心设施中，Markus Voehler或通过 与中心调查员或核心人员合作。在下一个赠款中 NMR设施的时期用途更重 病理学和酶促氧化和共轭研究核心。中心 贡献了中心人员累计的每小时使用费用的75％，更少的IS 由他们的学术单位做出贡献。该中心还提供一部分（15％） 斯通博士的薪水。该中心也为 购买新设备。总体而言，中心对实验室的贡献 运营费用直接取决于其利用 设备。