Synthesis and Study of Novel Sensing Agents

新型传感剂的合成与研究

• 批准号: 6520327
• 负责人: ROBERT M STRONGIN
• 金额: $ 18.38万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6520327
• 关键词: chemical synthesis; colorimetry; diagnosis design /evaluation; diagnostic tests; fructose; oligosaccharides; reagent /indicator; sialate; technology /technique development

DESCRIPTION: (Applicant's Description) This proposal addresses three important problem in biomedical science via the development of simple and rapid assays for biomolecules. The first goal involves the development of a room temperature color assay for fructose, a ubiquitous, rapidly metabolized sugar. The proposedassay is designed to be fructose-selective in the presence of a large excess of glucose. Fructose determination in plasma is currently hampered due to the large excess of glucose. The second goal involves creatinga mild assay for sialic acid, an important cell surface antigen, to address the problem of current assay inaccuracy based on hydrolytic procedures and/or relatively non-specific and harsh methods. The third goal entails the implementation of a new automated color detection system for oligosaccharides that does not require prior destructive hydrolysis or chemical labeling with a chromophore. Currently no detection technique exists for neutral oligosaccharides that is effective for the visual detection of oligosaccharides beyond three repeat units.

描述：（申请人的描述）该提案解决了三个重要的 生物医学科学中的问题通过开发简单和快速测定 用于生物分子。第一个目标涉及室温的发展 果糖的颜色测定，一种无处不在的，快速代谢的糖。这 拟议的纪念日设计为在大型的存在下是果糖选择性的 过量的葡萄糖。血浆中的果糖确定目前已妨碍 大量葡萄糖。第二个目标涉及创建温和测定 为了解决一个重要的细胞表面抗原，以解决 基于水解程序和/或相对的当前测定不准确性 非特异性和苛刻的方法。第三个目标需要实施 不需要的寡糖的新自动化颜色检测系统 先前用发色团的破坏性水解或化学标记。现在 对于有效的中性寡糖，不存在检测技术 为了直观地检测三个重复单元的寡糖。

项目成果

Phenotypic and genetic analysis of the cerebellar mutant tmgc26, a new ENU-induced ROR-alpha allele.

• DOI: 10.1111/j.1460-9568.2010.07330.x
• 发表时间: 2010-09
• 期刊: The European journal of neuroscience
• 作者: Swanson DJ;Steshina EY;Wakenight P;Aldinger KA;Goldowitz D;Millen KJ;Chizhikov VV
• 通讯作者: Chizhikov VV