GENOMIC ANALYSIS OF LAMBDOID, TB & OTHER BACTERIOPHAGES

LAMBDOID，TB 的基因组分析

• 批准号: 6525770
• 负责人: ROGER W HENDRIX
• 金额: $ 23.46万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-01-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6525770
• 关键词: bacterial virus; bacteriophage lambda; biochemical evolution; computer assisted sequence analysis; genetic polymorphism; nucleic acid sequence; technology /technique development; virus DNA; virus genetics

The dsDNA bacteriophages are the most numerous taxon of evolving organisms in the biosphere. However, their classification remains unclear and their inter-relationships are poorly understood. Characterization of these dsDNA phages by whole genome sequencing provide more detailed information of these relationships and provides an emerging picture in which essentially all dsDNA tailed bacteriophages have shared ancestry, even through they infect different hosts and have varied viral morphologies. Comparison of phage genomes also shows that they are constructed from a series of genetic modules that are joined into functional groups and they are essentially mosaic in nature. However, it is not known how many different modular segments there are, or how many variants exist of each module. Moreover, the mechanisms by which exchange occurs have yet to be clearly elucidated. We propose to investigate the process of phage evolution through the sequencing of phage genomes and comparisons of sequences, genome organization and function relationships. In addition, we propose to survey unselected phage populations in order to obtain a broader view of the diversity of bacteriophages that naturally exist in the environment. More sophisticated software systems will be developed that will greatly simplify the data analysis steps and provide automated methods for genome comparisons. These studies will not only lead to new insights into the mechanisms of viral evolution but will provide a valuable resource for other researchers, including those studying basic phage biology such as DNA replication, gene expression and macromolecular assembly as well as those interested in the application of phages to clinical problems, such as diagnosis and phage therapy.

dsDNA噬菌体是生物圈中不断发展的生物的最多分类单元。但是，他们的分类尚不清楚，他们的相互关系也很少理解。通过整个基因组测序对这些dsDNA噬菌体的表征提供了这些关系的更详细的信息，并提供了新的图片，其中基本上所有dsDNA尾巴噬菌体都具有祖先，即使通过它们感染了不同的宿主并具有不同的病毒形态。噬菌体基因组的比较还表明，它们是由一系列遗传模块构建的，这些模块与官能团相结合，并且本质上是马赛克。但是，尚不知道有多少个不同的模块化段，或每个模块存在多少个变体。此外，交换发生的机制尚未清楚地阐明。我们建议通过对噬菌体基因组的测序以及序列，基因组组织和功能关系的比较来研究噬菌体演变的过程。此外，我们建议调查未选择的噬菌体种群，以便更广泛地了解自然存在于环境中的噬菌体的多样性。将开发更复杂的软件系统，将大大简化数据分析步骤并为基因组比较提供自动化方法。这些研究不仅会导致对病毒进化机制的新见解，而且还将为其他研究人员提供宝贵的资源，包括研究基本噬菌体生物学的研究人员，例如DNA复制，基因表达和大分子分子组装，以及那些对将噬菌体应用于临床问题（例如诊断和噬菌体疗法）感兴趣的人。