GENOMIC ANALYSIS OF LAMBDOID, TB & OTHER BACTERIOPHAGES

LAMBDOID，TB 的基因组分析

• 批准号: 2634766
• 负责人: ROGER W HENDRIX
• 金额: $ 8.54万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-01-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2634766
• 关键词: bacterial virus; bacteriophage lambda; biochemical evolution; computer assisted sequence analysis; nucleic acid sequence; tuberculosis; virus genetics

The dsDNA bacteriophages are the most numerous taxon of evolving organisms in the biosphere. Information currently available from genomic sequencing of these organisms has revealed important insights into their evolution, and has given good reason to believe that a modest amount of additional sequence determination of whole genomes will substantially augment our understanding of the genetic structures of phage populations and of their evolutionary mechanisms and history. We propose to determine the sequences of nine bacteriophage genomes, three of the lambdoid family, four from the mycobacteriophages, and two others. We will analyze and compare the sequences to elucidate their genetic organizations and to make evolutionary inferences about the phages. In addition to providing evolutionary information for the bacteriophages, this project is expected to contribute to basic research on bacteriophage biology by providing comparative data about gene organization and function as well as by making new bacteriophages available for detailed experimentation. In addition, sequence data from this project is expected to assist in using bacteriophages to analyze and counteract human diseases caused by their hosts, specifically including tuberculosis.

dsDNA噬菌体是不断发展的生物的众多分类单元 在生物圈中。 目前可从基因组测序获得的信息 在这些生物中，揭示了对它们进化的重要见解， 并有充分的理由相信少量的额外 整个基因组的序列确定将大大增加我们的 了解噬菌体种群及其遗传结构 进化机制和历史。 我们建议确定 九个噬菌体基因组的序列，三个lambdoid家族， 分枝杆菌的四个，另外两个。 我们将分析和 比较序列以阐明其遗传组织并使 关于噬菌体的进化推论。 除了为噬菌体提供进化信息外， 预计该项目将有助于噬菌体的基础研究 通过提供有关基因组织和功能的比较数据来生物学 以及通过使新的噬菌体可用于详细 实验。 此外，有望来自该项目的序列数据 协助使用噬菌体分析和抵消人类疾病 由宿主引起的，特别是包括结核病。