PRODUCTION OF ANIMAL CELLS AND SECRETED PRODUCTS

动物细胞和分泌产物的生产

• 批准号: 6529781
• 负责人: Mark D. HIRSCHEL
• 金额: $ 41.55万
• 依托单位: BIOVEST INTERNATIONAL, INC.
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-21 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6529781
• 关键词: Baculoviridae; biological products; biomedical resource; biotechnology; cell bank /registry; cell line; clone cells; cooperative study; hybridomas; mass tissue /cell culture; monoclonal antibody; protein purification; recombinant proteins; transfection

DESCRIPTION (Adapted from the applicant's abstract): Mammalian cell culture- derived products, secreted proteins and cells, have developed into a critical component of ongoing biomedical research. The objective of this application is to continue the operation of the NCCC for large-scale animal cell services. Added technical support in the field of cell science helps to alleviate the current shortage of available facilities and expertise required to meet the cell culture needs of the research community. The application seeks continuation of the NCCC which is operated by full-time professional scientific and administrative staff that provides affordable services to the research community. The first Specific Aim of this application is to provide biomedical investigators throughout the United States of America (USA) with customized services for large quantity production of mammalian cells and secreted products. Services offered include: 1) production of hybridoma-secreted proteins, (monoclonal antibody); 2) production of recombinant or other anchorage-dependent cell-secreted proteins; 3) production of commonly used mammalian tumor or lymphoid cells; 4) custom production of specialized cell types; 5) baculovirus-infected insect cells; 6) transfection of investigator- derived DNA into established mammalian or insect cell lines; 7) a cell bank service; and 8) resource for large-scale cell culture advice. The second Specific Aim is to maintain a SAC with wide geographical distribution to assist in prioritization of user requests. This committee provides administrative assistance for implementation of investigator suggestions for improved services. The third Specific Aim is to maintain a system for tracking users of the Center's services and maintain close contact with the Biological Models and Materials Resources. The Center will continue to address the growing needs for professional cell culture services, both on a small- and large-scale. Professional services such as these will help satisfy the expanded research demands placed on the biomedical research community.

描述（改编自申请人的摘要）：哺乳动物细胞培养 - 衍生产品，分泌的蛋白质和细胞已发展为关键 正在进行的生物医学研究的组成部分。 此应用的目的 是继续进行NCCC的大规模动物细胞服务。 在细胞科学领域增加的技术支持有助于减轻 目前缺乏满足可用设施和专业知识 研究界的细胞培养需求。 申请寻求 由全职专业人员运营的NCCC的延续 科学和行政人员为 研究社区。 该应用程序的第一个具体目的是提供生物医学 全美的调查人员（美国）和定制 用于大量生产哺乳动物细胞并分泌的服务 产品。 提供的服务包括：1）杂交瘤分泌的生产 蛋白质（单克隆抗体）； 2）生产重组或其他 锚定依赖性细胞分泌蛋白； 3）常用的生产 哺乳动物肿瘤或淋巴样细胞； 4）定制生产专业单元格 类型； 5）杆状病毒感染的昆虫细胞； 6）转染研究者 - 将DNA衍生成已建立的哺乳动物或昆虫细胞系； 7）一个细胞库 服务; 8）用于大规模细胞培养建议的资源。 第二个特定目的是维持具有广泛地理的囊 分发以协助优先考虑用户请求。 这个委员会 为实施调查人员提供行政帮助 改进服务的建议。 第三个具体目的是维护跟踪用户的系统 中心的服务并与生物模型保持密切联系， 材料资源。 该中心将继续满足不断增长的专业牢房需求 文化服务，无论是在小规模和大规模上。 专业服务 诸如此类将有助于满足扩大的研究要求 生物医学研究界。