PRODUCTION OF ANIMAL CELLS AND SECRETED PRODUCT

动物细胞和分泌物的生产

• 批准号: 3560636
• 负责人: Mark D. HIRSCHEL
• 金额: $ 40.28万
• 依托单位: BIOVEST INTERNATIONAL, INC.
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-21 至 1995-09-20
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3560636
• 关键词: biotechnology; cell bank /registry; hybrid antibody; hybridomas; monoclonal antibody; neoplastic cell culture for noncancer research; protein engineering

Mammalian cell culture derived products, secreted proteins and cells, have developed into a critical component of on-going biomedical research. The objective of this proposal is to establish a National Cell Culture Center for contract animal cell production. Added technical support in the field of cell science will help alleviate the current shortage of available facilities and expertise required to meet the cell culture needs of the research community. This proposal seeks to establish a Cell Culture Center which will be operated by a full time professional scientific and administrative staff that will provide affordable services to the research community. 1. To provide biomedical investigators throughout the United States with customized service for large quantity production of mammalian cells and secreted products. Services to be offered include: a) production of hybridoma secreted proteins (monoclonal antibody), b) production of non- hybridoma or anchorage dependent secreted proteins, c) production of commonly used mammalian tumor or lymphoid cells, d) production of specialized cell types e.g. TIL and LAK cells, e) a cell bank service, f) growth feasibility and testing services which include mycoplasma and murine virus testing, g) production of non-pathogenic virus. 2. To establish a Scientific Advisory Committee with wide geographical distribution to assist in prioritization of user requests. This committee will provide administrative assistance for implementation of investigator suggestions for improved services. 3. To provide a system for tracking users of the Center's services and maintain close collaboration with the Biological Models and Materials Resources Section. The Center described above will address the growing need for professional cell culture services, both on a small and large scale. Professional services such as these will help satisfy the expanded research demands placed on the biomedical research community.

哺乳动物细胞培养的产物，分泌的蛋白质和细胞具有 发展为正在进行的生物医学研究的关键组成部分。 这 该提议的目的是建立一个国家细胞文化中心 用于合同动物细胞生产。 在现场增加了技术支持 细胞科学将有助于减轻当前可用的短缺 满足细胞文化需求所需的设施和专业知识 研究社区。 该建议旨在建立一个细胞培养中心 由全职专业科学和行政人员运营 这将为研究社区提供负担得起的服务。 1。为整个美国提供生物医学研究人员 用于大量生产哺乳动物细胞的定制服务和 分泌的产品。 要提供的服务包括：a）生产 杂交瘤分泌蛋白（单克隆抗体），b）产生非 - 杂交瘤或锚定依赖性分泌蛋白，c）产生 常用的哺乳动物肿瘤或淋巴样细胞，d）产生 专门的细胞类型，例如TIL和LAK细胞，E）一种细胞银行服务，f） 增长可行性和测试服务，包括支原体和 鼠病毒测试，g）产生非致病病毒。 2。建立一个科学咨询委员会，具有广泛的地理位置 分发以协助优先考虑用户请求。 这个委员会 将为实施调查员提供行政帮助 改进服务的建议。 3。提供一个系统来跟踪中心服务的用户和 与生物模型和材料保持密切的合作 资源部分。 上述中心将满足对专业人士的日益增长的需求 细胞培养服务，无论是小规模还是大规模的。 专业的 诸如此类的服务将有助于满足扩大的研究需求 放在生物医学研究界。