PRODUCTION OF ANIMAL CELLS AND SECRETED PRODUCTS

动物细胞和分泌产物的生产

• 批准号: 6078265
• 负责人: Mark D. HIRSCHEL
• 金额: $ 54.67万
• 依托单位: BIOVEST INTERNATIONAL, INC.
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-21 至 2000-09-20
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6078265
• 关键词: Baculoviridae; CHO cells; HeLa cells; biomedical resource; cell bank /registry; clone cells; cooperative study; hybridomas; monoclonal antibody; recombinant proteins; tissue /cell culture

Mammalian cell culture derived products, secreted proteins and cells, have developed into a critical component of ongoing biomedical research. The objective of this proposal is to continue the operation of the National Cell Culture Center for large-scale animal cell services. Added technical support in the field of cell science helps to alleviate the current shortage of available facilities and expertise required to meet the cell culture needs of the research community. This proposal seeks continuation of a Cell Culture Center which is operated by full time professional scientific and administrative staff that provides affordable services to the research community. The specific aims are as follows: 1. Provide biomedical investigators throughout the United States with customized services for large quantity production of mammalian cells and secreted products. Services offered include: a) production of hybridoma-secreted proteins (monoclonal antibody), b) production of recombinant or other anchorage dependent cell-secreted proteins, c) production of commonly used mammalian tumor or lymphoid cells, d) custom production of specialized cell types, 3) baculovirus infected insect cells, f) a cell bank service, g) resource for large-scale cell culture advice. 2. We intend to add to these research services a consulting and advisory service whereby the National Cell Culture Center will provide and assist clinical investigators in having their materials for clinical trials produced under GMP specifications. 3. Maintain a scientific advisory committee with wide geographical distribution to assist in priorization of user requests. This committee provides administraative assistance for implementation of investigator suggestions for improved services. 4. Maintain a system for tracking users of the center's services and maintain close contact with the Biological Models and Materials Resources Section. The Center will continue to address the growing needs for professional cell culture services, both on a small and large-scale. Professional services such as these will help satisfy the expanded research demands placed on the biomedical research community.

哺乳动物细胞培养产物，分泌的蛋白质和细胞， 已经发展成为正在进行的生物医学研究的关键组成部分。 该提议的目的是继续运营 国家大规模动物细胞服务国家细胞文化中心。 在细胞科学领域增加的技术支持有助于减轻 目前不需要的可用设施和专业知识 满足研究界的细胞培养需求。 该建议寻求延续一个细胞培养中心，这是 由全职专业科学和行政人员运营 这为研究社区提供了负担得起的服务。 这 具体目的如下：1。提供生物医学研究人员 在整个美国都有大量定制服务 产生哺乳动物细胞和分泌产品。 提供的服务 包括：a）产生杂交瘤分泌的蛋白质（单克隆 抗体），b）产生重组或其他锚定依赖性 细胞分泌的蛋白质，c）产生常用的哺乳动物肿瘤 或淋巴细胞，d）定制的专业细胞类型，3） 杆状病毒感染的昆虫细胞，f）细胞库服务，g）资源 有关大规模的细胞培养建议。 2。我们打算添加这些 研究服务咨询和咨询服务 细胞培养中心将提供并协助临床研究者 拥有他们的材料进行GMP制作的临床试验 规格。 3。维持一个科学咨询委员会 地理分布，以协助用户请求的优先级。 该委员会提供行政援助以实施 调查人员建议改进服务。 4。维护系统 用于跟踪中心服务的用户并保持密切联系 使用生物模型和材料资源部分。 中心 将继续满足不断增长的专业细胞文化需求 服务，无论是小规模和大型的服务。 专业服务 由于这些将有助于满足扩大的研究要求 生物医学研究界。