STRUCTURAL BASIS OF NUCLEOCYTOPLASMIC TRANSPORT

核质运输的结构基础

• 批准号: 6478931
• 负责人: CHRISTOPHER W AKEY
• 金额: $ 5.36万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6478931
• 关键词: biological products; biomaterials; biomedical resource; carbohydrates; immunology; infection; inflammation; lymphatic system; plants; spectrometry; structural biology

The protein glycosylation pathway still holds many questions in the case of lower eukaryotes. Thus, while the characteristics of some carbohydrate chains isolated from glycoproteins of certain filamentous fungi such as Aspergillus and Trichoderma resemble those found in yeast, others resemble those encountered in mammals. In an attempt to shed some light on the glycosylation in filamentous fungi, we have studied the predominant N-glycans from cellobiohydrolase 1, secreted from Trichoderma reesei RUTC 30. One of the compounds found is a novel phosphate-containing oligosaccharide that, to the best of our knowledge, has not been reported before. The primary structure of the novel phosphate-containing oligosaccharide, isolated from T reesei cellobiohydrolase 1, was determined by ES1 MS and found to comprise Glcl-6P03-Man7-9GlcNAC2. The Glu extensions appear to be on the lower 3-branched antenna. Thepresence of mannose residues linked through a phosphate diester resembles glycosyl synthesisin yeast. Several additional samples are under investigation.

蛋白质糖基化途径仍然存在许多问题 较低的真核生物的情况。 因此，虽然某些特征 从某些丝状糖蛋白分离的碳水化合物链 曲霉和trichoderma等真菌类似于发现的真菌 酵母，其他类似于哺乳动物遇到的人。 试图 在丝状真菌中阐明了糖基化的一些启示，我们有 研究了来自bobobiohydrollase 1的主要N-聚糖，分泌 来自Trichoderma Reesei rutc 30。发现的一种是 新颖的含磷酸盐的寡糖，这是我们最好的 知识，以前没有报道。 主要结构 从T reesei分离的新型含磷酸盐的寡糖 通过ES1 MS确定细胞胞胞胞成状水解酶1，并发现包含 GLCL-6P03-MAN7-9GLCNAC2。 GLU扩展名似乎在下部 3个分支天线。 通过A连接的甘露糖残留物的存在 磷酸盐二烯类似于糖基合成酵母。 一些 其他样本正在研究中。