STRUCTURE OF RM-Q COMPLEX

RM-Q复合物的结构

• 批准号: 8170588
• 负责人: CHRISTOPHER W AKEY
• 金额: $ 0.56万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170588
• 关键词: Burn injury; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Data Set; Engineering; Funding; Goals; Grant; Hospitals; Immune system; Institution; Lead; Legionella pneumophila; Legionnaires' Disease; Methionine; Molecular Chaperones; Mutation; Persons; Pneumonia; Proteins; Research; Research Personnel; Resolution; Resources; Source; Structure; System; Testing; Transplantation; United States National Institutes of Health; design; meetings; prevent; protein complex; ward

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Diffraction data will be collected from a complex of proteins that are critical to the function of a Type IVb secretion system in Legionella pneumophila. The T4bSS is required for infectivity and bacterial replication. Legionella pneumophila infections lead to a debilitating pneumonia that can be fatal in persons with a weakened immune system. This is particularly dangerous in hospital settings, such as transplant and burn wards. The crystals contain a 1:1 complex of the interacting region of IcmR, denoted Rm, and the full IcmQ proteins. The presence of IcmR likely blocks the function of IcmQ and acts as a chaperone to prevent aggregation. Our goal in this trip is to collect a higher resolution native data set (beyond 2.8A). The all alpha helical complex is ~24 kDa and we have engineered a complex with three Seleno-methionines. We will collect a multi-wavelength MAD dataset from Se-Met derivative crystals. This will allow us to solve the structure and then design mutations to test the function of this critical complex

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 衍射数据将从蛋白质复合物中收集，这对于肺炎军团菌中IVB型分泌系统的功能至关重要。 T4BS是感染性和细菌复制所必需的。 肺炎军团菌感染导致令人衰弱的肺炎，在免疫系统弱的人中可能致命。这在医院环境中尤其危险，例如移植和烧伤病房。晶体包含ICMR相互作用区域的1：1复合物，表示为RM和完整的ICMQ蛋白。 ICMR的存在可能会阻止ICMQ的功能，并充当防止聚集的伴侣。我们这次旅行的目标是收集更高的分辨率本机数据集（超出2.8a）。全α螺旋络合物构成约24 kDa，我们已经用三个硒蛋白来设计了一个复合体。 我们将从SE-MET导数晶体收集一个多波长的MAD数据集。这将使我们能够解决结构，然后设计突变以测试这种关键复合物的功能