Gene Specific Dna Repair

基因特异性 DNA 修复

• 批准号: 6530357
• 负责人: Vilhelm A Bohr
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6530357
• 关键词: DNA damage; DNA repair; Nematoda; aging; genetic transcription; nuclear matrix; radiation genetics; ultraviolet radiation

Summary of work: DNA repair can bes studied at the level of the whole genome, in specific regions, in specific genes, or in transcribed DNA. We have had an ongoing interest in the repair that takes place in genes and how it relates to gene function and cellular survival. We have examined the repair of essential genes and in various structural genes. We also examined whether repair was preferential in genes associated with the nuclear matrix. this appears to be the case as genes we have examined in that part of the cell structure are efficiently repaired. The nematode has been a very useful model system for the study of aging. There are useful mutants of many kinds including some with increased life span. These mutants with increased life span also are hyper resistant to various kinds of cellular damage, including UV irradiation and oxidative stress. By gene specific analysis of various gene regions in the nematode we seek to clarify whether the increased life span is associated with an increase in gene specific DNA repair

工作总结：DNA 修复可以在整个基因组水平、特定区域、特定基因或转录 DNA 水平上进行研究。我们一直对基因修复及其与基因功能和细胞存活的关系感兴趣。我们检查了必需基因和各种结构基因的修复。我们还检查了与核基质相关的基因是否优先修复。情况似乎就是这样，因为我们在细胞结构的该部分中检查过的基因得到了有效的修复。线虫一直是研究衰老的非常有用的模型系统。有多种有用的突变体，包括一些寿命更长的突变体。这些寿命延长的突变体也对各种细胞损伤具有超强的抵抗力，包括紫外线照射和氧化应激。通过对线虫各个基因区域进行基因特异性分析，我们试图阐明寿命的延长是否与基因特异性 DNA 修复的增加有关