Virus Host Interactions and Virion Assembly

病毒宿主相互作用和病毒粒子组装

• 批准号: 6506853
• 负责人: BERNARD MOSS
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6506853
• 关键词: cell cell interaction; confocal scanning microscopy; electron microscopy; host organism interaction; immunoelectron microscopy; laboratory mouse; laboratory rabbit; membrane proteins; membrane reconstitution /synthesis; polymerase chain reaction; protein protein interaction; protein sequence; recombinant virus; vaccinia virus; virion; virus assembly; virus envelope; virus genetics; virus infection mechanism; virus protein; virus replication

A productive infection by vaccinia virus involves entry into the cell; expression and replication of the genome; defense against specific and non-specific host immune mechanisms; and assembly and release of infectious, enveloped, virus particles. Many of these steps involve interactions of viral proteins with cellular proteins or membranes. Combined genetic, biochemical, and electron microscopic approaches are being used to investigate these complex processes. During the past year, emphasis has been on understanding the steps in the formation and transmission of intracellular and extracellular virions, the host range restriction of poxviruses, and viral immune defense proteins.

离甲酸病毒的生产性感染涉及进入细胞。基因组的表达和复制；防御特定和非特异性宿主免疫机制；以及传染性，包膜，病毒颗粒的组装和释放。这些步骤中的许多步骤涉及病毒蛋白与细胞蛋白或膜的相互作用。遗传，生化和电子显微镜方法的组合用于研究这些复杂过程。在过去的一年中，重点是理解细胞内和细胞外病毒体的形成和传播，宿主范围的限制以及病毒免疫防御蛋白的限制。