REGULATION OF VACCINIA VIRUS GENE EXPRESSION

痘苗病毒基因表达的调控

• 批准号: 6288824
• 负责人: BERNARD MOSS
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6288824
• 关键词: Baculoviridae; electron microscopy; gene expression; gene targeting; genetic promoter element; genetic regulation; laboratory rabbit; open reading frames; recombinant DNA; recombinant virus; tissue /cell culture; transcription factor; vaccinia virus; virus genetics; virus infection mechanism

Poxviruses encode enzymes and factors needed for transcription of their genomes within the cytoplasm of infected cells. Vaccinia virus, the prototypic member of the poxvirus family, provides a unique system for combining biochemical and genetic approaches for investigating mechanisms of gene regulation and mRNA biosynthesis. Studies with vaccinia virus indicated that the genes are divided into three temporal classes - early, intermediate and late. Each gene class has a consensus DNA promoter sequence and corresponding transcription factors that interact with the virus-encoded multisubunit RNA polymerase. The transcription system for early genes is packaged within the infectious virus particle during its assembly, whereas the factors for intermediate and late gene transcription are synthesized successively after infection. - Poxvirus, vaccinia virus, transcription, DNA replication

痘病毒编码在感染细胞的细胞质内转录其基因组所需的酶。痘病毒家族的原型成员Vaccinia病毒提供了一个独特的系统，用于结合生化和遗传方法，以研究基因调节和mRNA生物合成机制。对离甲酸病毒的研究表明，这些基因分为三个时间类别 - 早期，中间和晚期。每个基因类具有共有的DNA启动子序列和相应的转录因子，这些转录因子与病毒编码的多亚基RNA聚合酶相互作用。早期基因在组装过程中的转录系统被包装在传染病颗粒中，而感染后中间基因转录的因子和晚期基因转录的因素是连续合成的。 - 痘病毒，离发病毒，转录，DNA复制