Poxvirus Entry

痘病毒进入

• 批准号: 7732469
• 负责人: BERNARD MOSS
• 金额: $ 93.73万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7732469
• 关键词: Cell membrane; Cells; Complex; Endocytosis; Family; Goals; Membrane; Membrane Fusion; Poxviridae; Proteins; Reagent; Vaccinia virus; Virus; Virus Replication; member; mutant; prototype; tissue/cell culture

The first step in virus replication is entry. The mechanisms used by poxviruses are complicated by the existence of two distinct infectious forms with different external membranes. Another complicating factor is the ability of vaccinia virus to enter cells through the plasma membrane and by endocytosis. We have identified a complex comprised of 8 proteins that are required for entry of both virus forms. These proteins are conserved in all members of the poxvirus family, suggesting a common entry mechanism.

病毒复制的第一步是进入。痘病毒所使用的机制由于存在两种不同的外部膜的不同传染性形式而变得复杂。另一个复杂的因素是离子病毒通过质膜和内吞作用进入细胞的能力。我们已经确定了一个由8种蛋白质组成的复合物，这些蛋白需要进入两种病毒形式。这些蛋白质在Poxvirus家族的所有成员中都是保守的，这表明是一种共同的进入机制。

项目成果

A viral member of the ERV1/ALR protein family participates in a cytoplasmic pathway of disulfide bond formation.

• DOI: 10.1073/pnas.210397997
• 发表时间: 2000-10
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: T. Senkevich;C. L. White;E. Koonin;B. Moss

Molluscum contagiosum virus interleukin-18 (IL-18) binding protein is secreted as a full-length form that binds cell surface glycosaminoglycans through the C-terminal tail and a furin-cleaved form with only the IL-18 binding domain.

传染性软疣病毒白细胞介素 18 (IL-18) 结合蛋白以全长形式分泌，通过 C 末端尾部结合细胞表面糖胺聚糖，以及仅具有 IL-18 结合结构域的弗林蛋白酶切割形式。

• DOI: 10.1128/jvi.77.4.2623-2630.2003
• 发表时间: 2003
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Xiang,Yan;Moss,Bernard
• 通讯作者: Moss,Bernard

Mapping and functional analysis of interaction sites within the cytoplasmic domains of the vaccinia virus A33R and A36R envelope proteins.

痘苗病毒 A33R 和 A36R 包膜蛋白胞质域内相互作用位点的定位和功能分析。

• DOI: 10.1128/jvi.77.7.4113-4126.2003
• 发表时间: 2003
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Ward,BrianM;Weisberg,AndreaS;Moss,Bernard
• 通讯作者: Moss,Bernard

Vaccinia virus A30L protein is required for association of viral membranes with dense viroplasm to form immature virions.

痘苗病毒 A30L 蛋白是病毒膜与致密病毒质结合形成未成熟病毒粒子所必需的。

• DOI: 10.1128/jvi.75.13.5752-5761.2001
• 发表时间: 2001
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Szajner,P;Weisberg,AS;Wolffe,EJ;Moss,B
• 通讯作者: Moss,B

Unique temperature-sensitive defect in vaccinia virus morphogenesis maps to a single nucleotide substitution in the A30L gene.

痘苗病毒形态发生中独特的温度敏感缺陷归因于 A30L 基因中的单核苷酸取代。

• DOI: 10.1128/jvi.75.22.11222-11226.2001
• 发表时间: 2001
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Szajner,P;Weisberg,AS;Moss,B
• 通讯作者: Moss,B