CORE--TRANSGENIC ANIMALS

核心——转基因动物

• 批准号: 6442964
• 负责人: PAUL P WONG
• 金额: $ 11.79万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6442964
• 关键词: animal breeding; animal colony; biomedical facility; disease /disorder model; environmental health; environmental toxicology; fish; gene targeting; genetic strain; genetically modified animals; laboratory mouse; microinjections

Description: The Transgenic Animals Core provides CRED members the specialized equipment and technical assistant needed in the development of transgenic mouse models via DNA microinjection. In addition, this Core offers assistance in deriving pathogen-free mouse strains via embryo transfer. The Core is currently developing procedures for embryo freezing and storage. Plans include the development of a transgene plasmid library to facilitate member development of transgenic mouse models and the introduction of additional mouse strains as egg donors for DNA microinjection. The Core tentatively intends to offer assistance in generating mouse embryonic stem cell derived knock-out, knock-in, and conditional knock-out mice in the next five-year period. A new focus of the Core will be the production of transgenic fish in collaboration Dr. Nairn (Director, RC2), Dr. Bailey of the Marine/Freshwater Biomedical Sciences Center at Oregon State University, and Dr. Richard Winn at the University of Georgia.

描述：转基因动物核心提供信用成员 在开发中需要的专业设备和技术助理 通过DNA显微注射的转基因小鼠模型。此外，此核心提供 通过胚胎转移得出无病原体小鼠菌株的帮助。这 核心目前正在开发胚胎冷冻和存储的程序。 计划包括开发转基因质粒库以促进 转基因小鼠模型的成员开发和引入 其他小鼠菌株作为DNA显微注射的卵子供体。核心 暂时打算在产生小鼠胚胎词干方面提供帮助 细胞衍生的敲除，敲门和有条件的淘汰小鼠 五年。核心的新重点将是生产 转基因鱼在合作中 俄勒冈州立大学的海洋/淡水生物医学中心， 佐治亚大学的理查德·温恩博士。