EXCITOTOXIC & NEURONAL DYSFUNCTION IN BRAIN INJURY

兴奋性毒性

• 批准号: 6477596
• 负责人: DAVID A HOVDA
• 金额: $ 4.85万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6477596
• 关键词: Chordata; Mammalia; animal tissue; bioimaging /biomedical imaging; biomedical resource; computers; magnetic resonance imaging; model design /development; nervous system; nuclear magnetic resonance spectroscopy; statistics /biometry

The long-term goal of this research is to build a better bridge between the tissue, cellular, and molecular levels of analysis of the frog embryo. The specific goal of this proposal is to probe the nature of molecular expression domains in the early embryo. The ability of pMRI to follow tissue movements and cell lineages in three dimensions over time in opaque specimens will be used, in conjunction with conventional molecular biology techniques, to map the lineages that contribute to a gene expression domain as well as the fates of the cells that were once within the domain. The specific hypothesis to be tested is that the gene expression domains are made up of dynamic populations of cells, requiring that cells express the marker gene as they enter a given domain, and extinguish it as they exit the domain. Towards this end, we will: (1) determine the normal lineages of cells that contribute to the frog nervous system. the axial mesoderm and the paraxial mesoderm: (2) determine the rel ationship between the early lineages and differential patterns of gene expression: (3) determine the changes in cell lineages and cell movements in perturbed embryos 1. Goal Directed Imaging: (a) provide comprehensive three-dimensional in vivo images of the developing brain in three different species (quail, mouse, and monkey) using Magnetic Resonance (MR) micro-imaging, (b) use fMRI techniques to examine how the visual field map of the owl monkey changes with increasing degree of myelination in brain of the young animal. 2. Analysis: (a) analyze the images at a number of different levels ranging from simple annotation to correlating the onset and developing organization of different structures in both space & time and to compare & contrast the avian, rodent, and mammalian systems. (b) mesh optical and MRI data of the same animal. 3. Dissemination: (a) make the images and tools for rendering & analysis available to the scientific community in a convenient format. e.g., photographs of 2D slices of the 3D data. CD-ROMs with annotated 2D and 3D images to be viewed using readily available software and software developed in-house. and an on-line database accessible via the World Wide Web where raw' as well as 'processed' data will potentially be available.

这项研究的长期目标是建造更好的桥梁 在组织，细胞和分子之间的分析之间 青蛙胚胎。 该提案的具体目标是探究 早期胚胎中分子表达结构域的性质。 这 PMRI跟随组织运动和三个细胞谱系的能力 将使用不透明标本的尺寸随着时间的流逝而结合使用 使用常规的分子生物学技术，以绘制谱系 这有助于基因表达域以及 曾经在域内的细胞。 特定的假设 要测试的是基因表达域是由 细胞的动态种群，要求细胞表达标记 当他们进入一个给定的领域时，当他们退出时将其扑灭 领域。 在这一目标中，我们将：（1）确定正常谱系 有助于青蛙神经系统的细胞。 轴向 中胚层和近期中胚层：（2）确定相关性 在早期谱系和基因差异模式之间 表达式：（3）确定细胞谱系和细胞的变化 扰动胚胎的运动 1。目标成像：（a）提供全面 三维发展大脑的三维体内图像 使用磁共振的不同物种（鹌鹑，小鼠和猴子） （MR）微成像，（b）使用fMRI技术来检查视觉 猫头鹰猴的现场图随着程度的增加而变化 年轻动物大脑中的髓鞘化。 2。分析：（a）以多种不同级别分析图像 从简单的注释到开始发作和开发 在空间和时间和时间内组织不同结构的组织 比较和对比禽，啮齿动物和哺乳动物系统。 （b）网格 同一动物的光学和MRI数据。 3。传播：（a）制作渲染图像和工具 科学界以方便的格式可提供分析。 例如，3D数据的2D切片的照片。 带注释的CD-ROM 使用随时可用的软件查看的2D和3D图像， 软件在内部开发。 以及可以通过在线数据库访问 RAW和“处理”数据的万维网将 有可能可用。