EXCITOTOXIC & NEURONAL DYSFUNCTION IN BRAIN INJURY

兴奋性毒性

• 批准号: 6477596
• 负责人: DAVID A HOVDA
• 金额: $ 4.85万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6477596
• 关键词: Chordata; Mammalia; animal tissue; bioimaging /biomedical imaging; biomedical resource; computers; magnetic resonance imaging; model design /development; nervous system; nuclear magnetic resonance spectroscopy; statistics /biometry

The long-term goal of this research is to build a better bridge between the tissue, cellular, and molecular levels of analysis of the frog embryo. The specific goal of this proposal is to probe the nature of molecular expression domains in the early embryo. The ability of pMRI to follow tissue movements and cell lineages in three dimensions over time in opaque specimens will be used, in conjunction with conventional molecular biology techniques, to map the lineages that contribute to a gene expression domain as well as the fates of the cells that were once within the domain. The specific hypothesis to be tested is that the gene expression domains are made up of dynamic populations of cells, requiring that cells express the marker gene as they enter a given domain, and extinguish it as they exit the domain. Towards this end, we will: (1) determine the normal lineages of cells that contribute to the frog nervous system. the axial mesoderm and the paraxial mesoderm: (2) determine the rel ationship between the early lineages and differential patterns of gene expression: (3) determine the changes in cell lineages and cell movements in perturbed embryos 1. Goal Directed Imaging: (a) provide comprehensive three-dimensional in vivo images of the developing brain in three different species (quail, mouse, and monkey) using Magnetic Resonance (MR) micro-imaging, (b) use fMRI techniques to examine how the visual field map of the owl monkey changes with increasing degree of myelination in brain of the young animal. 2. Analysis: (a) analyze the images at a number of different levels ranging from simple annotation to correlating the onset and developing organization of different structures in both space & time and to compare & contrast the avian, rodent, and mammalian systems. (b) mesh optical and MRI data of the same animal. 3. Dissemination: (a) make the images and tools for rendering & analysis available to the scientific community in a convenient format. e.g., photographs of 2D slices of the 3D data. CD-ROMs with annotated 2D and 3D images to be viewed using readily available software and software developed in-house. and an on-line database accessible via the World Wide Web where raw' as well as 'processed' data will potentially be available.

这项研究的长期目标是建造一座更好的桥梁 组织、细胞和分子水平之间的分析 青蛙胚胎。 该提案的具体目标是探讨 早期胚胎中分子表达域的性质。 这 pMRI 能够跟踪三种组织运动和细胞谱系 不透明样本随时间变化的尺寸将结合使用 利用传统的分子生物学技术绘制谱系图 有助于基因表达域以及命运 曾经在域内的细胞。 具体假设 要测试的是基因表达域由以下组成 动态细胞群，要求细胞表达标记 当它们进入给定域时基因，并在它们退出特定域时消除它 领域。 为此，我们将：（1）确定正常谱系 对青蛙神经系统有贡献的细胞。 轴向 中胚层与近轴中胚层：(2)确定关系 早期谱系和基因差异模式之间 表达式：(3)判断细胞谱系和细胞的变化 受干扰胚胎的运动 1. 目标定向成像：(a) 提供全面的 发育中大脑的三维活体图像 使用磁共振分析不同物种（鹌鹑、小鼠和猴子） (MR) 显微成像，(b) 使用功能磁共振成像技术来检查视觉 猫头鹰猴的场图随着程度的增加而变化 幼小动物大脑中的髓鞘形成。 2. 分析： (a) 在多个不同级别上分析图像 从简单的注释到关联发病和发展 在空间和时间上组织不同的结构 比较和对比鸟类、啮齿动物和哺乳动物系统。 (b) 网格 同一动物的光学和 MRI 数据。 3. 传播： (a) 制作用于渲染的图像和工具 以方便的格式向科学界提供分析。 例如，3D 数据的 2D 切片照片。 带注释的 CD-ROM 使用现成的软件可以查看 2D 和 3D 图像， 内部开发的软件。 以及可通过以下方式访问的在线数据库 万维网，原始数据和处理后的数据都将在其中 可能可用。