CORE--ANIMAL MODELING AND OUTCOME

核心——动物建模和结果

• 批准号: 6565232
• 负责人: PATRICK M KOCHANEK
• 金额: $ 20.73万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6565232
• 关键词: biomedical facility; brain injury; disease /disorder model; experimental brain lesion; induced hypothermia; laboratory mouse; laboratory rat; magnetic resonance imaging; model design /development; nervous system disorder therapy; biomedical facility; brain injury; disease /disorder model; experimental brain lesion; induced hypothermia; laboratory mouse; laboratory rat; magnetic resonance imaging; model design /development; nervous system disorder therapy

We propose to operate a core facility that performs the necessary procedures related to Animal Modeling and Outcome Assessment for the University of Pittsburgh Brain Trauma Research Center program project grant (PPG). The facilities in this core will be used by the following principal investigators (PIs) involved in the individual proposals in this PPG application: 1) Steven Graham, M.D., Ph.D., Project #1, "bcl-2 family genes in traumatic brain injury (TBI)", 2) Steven Dekosky., Project $2, "IL-1beta, APP metabolism and hypothermia in head injury", 3) Patrick Kochanek, M.D., Project #3, "iNOS and TBI", 4) Edward Dixon, Ph.D., Project #4 "Dopaminergic mechanisms of TBI," and 5) Robert Clark, M.D., Project #5 "PARS activation after TBI." The core facility will address the following Specific Aims 1) To provide a centralized facility for surgery and anesthesia for all of the proposed experimental manipulations of mice and rats within the PPG, 2) To provide uniform injury for all studies in mice and rats in the PPG (controlled cortical impact [CI] model, and CCI model with secondary insult) and ensure consistency by performing quality control analysis, 3) To provide a centralized facility for the standardized assessment of functional outcome parameters (motor and cognitive) within the PPG, 4) To provide a centralized facility for the standardized assessment of histological outcome parameters (contusion volume and hippocampal neuron counting) within the PPG, 5) To facilitate interaction between the PIs within the PPG, and facilitate interaction with collaborating investigators at the Pittsburgh NMR Center for Biomedical Research (Carnegie Mellon University), and 6) To provide a standardized hypothermia regimen for all studies using this therapy in rats in the PPG. The successful execution of these specific aims will provide for optimal resource utilization by the investigators include the use of 1) technician time, 2) laboratory space, 3) equipment, 4) supplies, 5) animals, and 6) Magnetic Resonance Imaging time. At the same time, quality control will be ensured for all of the studies in mice and rats, and a structured environment for interaction between the PIs within the PPG will be provided.

我们建议操作一个核心设施，该设施执行与匹兹堡大学脑创伤研究中心计划项目赠款（PPG）的动物建模和结果评估相关的必要程序。该核心中的设施将由以下主要研究人员（PI）使用此PPG应用程序中的个人建议：1）史蒂文·格雷厄姆（Steven Graham）医学博士Kochanek，项目＃3，“ Inos and TBI”，4）Edward Dixon博士，Project＃4“ TBI的多巴胺能机制”和5）Robert Clark，M.D. The core facility will address the following Specific Aims 1) To provide a centralized facility for surgery and anesthesia for all of the proposed experimental manipulations of mice and rats within the PPG, 2) To provide uniform injury for all studies in mice and rats in the PPG (controlled cortical impact [CI] model, and CCI model with secondary insult) and ensure consistency by performing quality control analysis, 3) To provide a centralized facility for the standardized assessment of functional outcome parameters (motor and cognitive) within the PPG, 4) To provide a centralized facility for the standardized assessment of histological outcome parameters (contusion volume and hippocampal neuron counting) within the PPG, 5) To facilitate interaction between the PIs within the PPG, and facilitate interaction with collaborating investigators at the Pittsburgh NMR Center for Biomedical Research （卡内基·梅隆大学）和6）为在PPG中使用这种疗法的所有研究提供标准化的体温过低方案。这些特定目标的成功执行将提供研究人员的最佳资源利用，包括使用1）技术员时间，2）实验室空间，3）设备，4）供应，5）动物和6）磁共振成像时间。同时，将确保对小鼠和大鼠的所有研究确保质量控制，并将提供PPG内PI之间相互作用的结构化环境。