CORE--ANIMAL MODELING AND OUTCOME

核心——动物建模和结果

• 批准号: 6448238
• 负责人: PATRICK M KOCHANEK
• 金额: $ 20.73万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6448238
• 关键词: biomedical facility; brain injury; disease /disorder model; experimental brain lesion; induced hypothermia; laboratory mouse; laboratory rat; magnetic resonance imaging; model design /development; nervous system disorder therapy

We propose to operate a core facility that performs the necessary procedures related to Animal Modeling and Outcome Assessment for the University of Pittsburgh Brain Trauma Research Center program project grant (PPG). The facilities in this core will be used by the following principal investigators (PIs) involved in the individual proposals in this PPG application: 1) Steven Graham, M.D., Ph.D., Project #1, "bcl-2 family genes in traumatic brain injury (TBI)", 2) Steven Dekosky., Project $2, "IL-1beta, APP metabolism and hypothermia in head injury", 3) Patrick Kochanek, M.D., Project #3, "iNOS and TBI", 4) Edward Dixon, Ph.D., Project #4 "Dopaminergic mechanisms of TBI," and 5) Robert Clark, M.D., Project #5 "PARS activation after TBI." The core facility will address the following Specific Aims 1) To provide a centralized facility for surgery and anesthesia for all of the proposed experimental manipulations of mice and rats within the PPG, 2) To provide uniform injury for all studies in mice and rats in the PPG (controlled cortical impact [CI] model, and CCI model with secondary insult) and ensure consistency by performing quality control analysis, 3) To provide a centralized facility for the standardized assessment of functional outcome parameters (motor and cognitive) within the PPG, 4) To provide a centralized facility for the standardized assessment of histological outcome parameters (contusion volume and hippocampal neuron counting) within the PPG, 5) To facilitate interaction between the PIs within the PPG, and facilitate interaction with collaborating investigators at the Pittsburgh NMR Center for Biomedical Research (Carnegie Mellon University), and 6) To provide a standardized hypothermia regimen for all studies using this therapy in rats in the PPG. The successful execution of these specific aims will provide for optimal resource utilization by the investigators include the use of 1) technician time, 2) laboratory space, 3) equipment, 4) supplies, 5) animals, and 6) Magnetic Resonance Imaging time. At the same time, quality control will be ensured for all of the studies in mice and rats, and a structured environment for interaction between the PIs within the PPG will be provided.

我们建议运营一个核心设施，为匹兹堡大学脑创伤研究中心项目拨款（PPG）执行与动物建模和结果评估相关的必要程序。该核心设施将由参与本 PPG 申请中各个提案的以下主要研究者 (PI) 使用：1) Steven Graham，医学博士、博士，项目 #1，“创伤性创伤中的 bcl-2 家族基因”脑损伤 (TBI)”，2) Steven Dekosky.，项目 $2，“头部损伤中的 IL-1beta、APP 代谢和体温过低”，3) Patrick Kochanek，医学博士，项目 #3， “iNOS 和 TBI”，4) Edward Dixon 博士，项目 #4“TBI 的多巴胺能机制”，以及 5) Robert Clark 医学博士，项目 #5“TBI 后的 PARS 激活”。核心设施将实现以下具体目标 1) 为 PPG 内所有拟议的小鼠和大鼠实验操作提供集中的手术和麻醉设施，2) 为 PPG 内小鼠和大鼠的所有研究提供统一的损伤PPG（受控皮质影响 [CI] 模型和具有继发性损伤的 CCI 模型）并通过执行质量控制分析确保一致性，3) 为 PPG 内功能结果参数（运动和认知）的标准化评估提供集中设施， 4）为 PPG 内组织学结果参数（挫伤体积和海马神经元计数）的标准化评估提供集中设施，5) 促进 PPG 内 PI 之间的互动，并促进与匹兹堡 NMR 生物医学中心合作研究人员的互动研究（卡内基梅隆大学），以及 6) 为所有在 PPG 大鼠中使用这种疗法的研究提供标准化的低温治疗方案。这些具体目标的成功执行将为研究人员提供最佳的资源利用，包括使用 1) 技术人员时间、2) 实验室空间、3) 设备、4) 用品、5) 动物和 6) 磁共振成像时间。同时，将确保所有小鼠和大鼠研究的质量控制，并为 PPG 内 PI 之间的相互作用提供结构化环境。