MOLECULAR BASIS FOR VERTEBRATE CELL MOTILITY

脊椎动物细胞运动的分子基础

• 批准号: 6592830
• 负责人: Jim Jung-Ching Lin
• 金额: $ 14.32万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-16 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6592830
• 关键词: CHO cells; Drosophilidae; actins; caldesmon; cell biology; cell cycle; cell motility; collagen; computer simulation; digital imaging; green fluorescent proteins; immunofluorescence technique; laboratory mouse; laboratory rabbit; microfilaments; monoclonal antibody; mutant; myosins; phosphorylation; protein isoforms; protein protein interaction; protein structure function; tissue /cell culture; tropomyosin

Description (provided in specific aims by applicant): Our long term goal is to understand how nonmuscle cells move and divide. Actin and actin motors provide the driving force for nonmuscle cell motility and cytokinesis, and a variety of actin-binding proteins regulate this process. However, the manner in which actin-binding proteins accomplish this is poorly understood. My major interest has been in the role of the tropomyosin isoforms and the proteins that interact with the tropomyosins, including caldesmon, tropomodulin, Endostatin and myosin Va. In the next Program period, I will continue to explore how these molecules ineract to effect cell motility and cytokineses. This project aims to study the roles of tropomyosin isoforms and their interacting proteins in regulating the motility and cytokinesis of nonmuscle cells. These proposed studies will combine molecular, biochemical and cell biological approaches together with computer-assisted reconstruction and motion analysis to investigate the functions of caldesmon phosphorylation sites, tropomyosin isoforms, tropomyosin interactions with tropomodulin and with endostatin, and functions of myosin Va.

描述（由申请人提供具体目标）：我们的长期目标是 了解非肌肉细胞如何移动和分裂。肌动蛋白和肌动蛋白马达 为非肌细胞运动和胞质分裂提供驱动力，并 多种肌动蛋白结合蛋白调节这一过程。 然而，方式 肌动蛋白结合蛋白是如何实现这一目标的，人们知之甚少。我的 主要兴趣在于原肌球蛋白亚型的作用和 与原肌球蛋白相互作用的蛋白质，包括钙结合蛋白， 原调节蛋白、内皮抑素和肌球蛋白 Va。在下一个计划期间，我将 继续探索这些分子如何相互作用来影响细胞运动和 细胞因子。 该项目旨在研究原肌球蛋白亚型及其作用 调节非肌肉运动和胞质分裂的相互作用蛋白 细胞。这些拟议的研究将结合分子、生物化学和细胞 生物学方法与计算机辅助重建和 运动分析研究卡尔德斯蒙磷酸化的功能 位点、原肌球蛋白亚型、原肌球蛋白与原调节蛋白的相互作用以及 具有内皮抑素和肌球蛋白 Va 的功能。