Polyurethane Biomaterials with Heparin Binding Sites

具有肝素结合位点的聚氨酯生物材料

• 批准号: 6550259
• 负责人: ROBERT S WARD
• 金额: $ 10万
• 依托单位: POLYMER TECHNOLOGY GROUP, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6550259
• 关键词: antithrombogenic surface; atomic force microscopy; binding sites; biomaterial development /preparation; biomaterial evaluation; heparin; polyurethanes; spectrometry

DESCRIPTION (provided by applicant):It is well known that the thrombo-resistance of polymeric biomaterials can be improved by surface endpoint immobilization of heparin. Current heparinization methods are complex and costly, and may degrade the mechanical properties of the base polymer. The proposed study will determine the feasibility of synthesizing tough, thermoplastic polyurethane biomaterials with 'built-in' binding sites for heparin. Heparinization will be achieved by simply soaking the device/component made from the subject polymer, in an aqueous heparin solution. A diamine-diamide-diol (PIME), which has an established ability to bind heparin, will replace some of the normal diol 'chain extender' in a well-established biomedical polycarbonate-urethane. Surfaces of the resulting PIME-containing polyurethanes will be characterized by Sum Frequency Generation Spectroscopy (SFG), Contact Angle Goniometry, and Atomic Force Microscopy. Heparin activity will be determined by Activated Partial Thromboplastic Time (APTT) on freshly synthesized and sterilized device analogues. Preliminary results indicate surface PIME can be easily distinguished from the base polyurethane by SFG. Also, non-sterile heparinized PIME-containing polyurethanes exhibit an APTT of >500 seconds compared to <55 seconds for polyurethanes without PIME. Biomaterials with inherent affinity for heparin will reduce the cost of surface heparinization and are therefore applicable to a wide range of blood-contact medical devices and prostheses.

描述（由申请人提供）：众所周知，可以通过脱离肝素的表面端点固定来改善聚合物生物材料的血栓抗性。当前的肝素化方法是复杂且昂贵的，并且可能会降解碱基聚合物的机械性能。拟议的研究将确定与肝素的“内置”结合位点合成坚固的热塑性聚氨酯生物材料的可行性。通过简单地将主体聚合物制成的设备/组件（在水溶液溶液中）来实现肝素化。具有结合肝素的能力的二胺二胺二醇（PIME）将在建立良好的生物医学聚碳酸酯 - 尿硫烷中取代一些正常的Diol“链扩展器”。所得的含PIME聚氨酯的表面将以总和频率产生光谱（SFG），接触角性角度计和原子力显微镜为特征。肝素活性将通过新鲜合成和消毒的装置类似物上的激活部分血栓形成时间（APTT）确定。初步结果表明，SFG可以很容易地将表面PIME与碱基聚氨酯区分开。同样，非肝素化聚氨酯的非脱甲基化聚氨酯的APTT> 500秒，而没有PIME的聚氨酯<55秒。对肝素的固有亲和力的生物材料将降低表面肝素化的成本，因此适用于广泛的血液接触医疗设备和假体。