TESTICULAR NERVOUS SYSTEM OF PRIMATES

灵长类动物的睾丸神经系统

• 批准号: 6453664
• 负责人: HENRYK F URBANSKI
• 金额: $ 11.11万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6453664
• 关键词: Mammalia; endocrine gland /system; growth /development; nervous system; reproductive system

Intrinsic neuron-like cells expressing the catecholamine-biosynthetic enzyme tyrosine hydroxylase (TH) have recently been identified in the testis of the prepubertal rhesus monkey. The aims of this study were to characterize the neuron-like nature of these cells, and to examine the distribution and frequency of testicular neuronal elements during postnatal development, puberty, adulthood and old age. Using immunohistochemistry, nerve fibers and cell bodies immunoreactive for the neuronal markers neurofilament 200 (NF-200) and synaptosomal associated protein of 25 kDa (SNAP 25), TH and neuropeptide Y, were identified in perivascular locations, intermingled with interstitial Leydig cells and close to the wall of seminiferous tubules. Moreover, marked age-related differences in the numbers of these neuronal elements became apparent when the NF-200 immunoreactive neuronal elements were quantified. Thus, intrinsic neuron-like cell bodies were found only in the testes fro m immatu re animals (<3 years of age). Conversely, nerve fibers were observed at all ages, although they became more prominent after the pubertal increase in LH and testosterone levels. Interestingly, mast cells, which are known to contain potent regulatory substances, were found in close anatomical proximity to nerve fibers. The number of these cells, positively identified with an antibody to tryptase, increased significantly after puberty following the same developmental pattern as the nerve fibers. These results confirm that the testicular nervous system of the monkey comprises two components, intrinsic nerve cells and extrinsic fibers, both of which are catecholaminergic and peptidergic in nature. Furthermore, both components are highly plastic during development, suggesting a possible participation in the onset of puberty. The intratesticular locations of neuron-like cells and fibers suggest that catecholamines and neuropeptides may have multiple sites of actions, and may affect Leydig cel ls, cells of the tubular wall, and vascular cells directly and/or indirectly via intermediation of mast cells. FUNDING Nonfederal Institutional funds PUBLICATIONS Lue YH, Sinhahikim AP, Machida Y, Mayerhofer A, Urbanski HF, Wang C, Swedloff RS. Accelerated germ cell apoptosis in aging rhesus monkeys. In Endocrine Society Program & Abstracts 80th Annual Meeting (held in New Orleans, LA, June 24-27, 1998), p 381, 1998 (abstract). Frunigieri M, Urbanski HF, Calandra R, Mayerhofer A. Elmentos neuronales testiculares en el mono. Medicina Buenos Aires 58:438, 1998 (abstract).

内源性神经元样细胞表达 儿茶酚胺生物合成酶酪氨酸羟化酶 (TH) 最近在青春期前恒河猴的睾丸中发现 猴。 本研究的目的是表征神经元样 这些细胞的性质，并检查分布和频率 出生后发育、青春期睾丸神经元元件 成年期和老年期。 使用免疫组织化学、神经纤维和 对神经元标记物神经丝 200 具有免疫反应的细胞体 (NF-200) 和 25 kDa 突触体相关蛋白 (SNAP 25)，TH 和神经肽 Y，在血管周围位置被鉴定， 与间质 Leydig 细胞混合并靠近细胞壁 生精小管。 此外，与年龄相关的显着差异 当 NF-200 出现时，这些神经元元件的数量变得明显 对免疫反应性神经元元件进行定量。 因此，内在的 神经元样细胞体仅在未成熟的睾丸中发现 动物（<3 岁）。 相反，在以下位置观察到神经纤维： 所有年龄段，尽管在青春期后变得更加突出 LH 和睾酮水平增加。 有趣的是，肥大细胞， 已知含有有效的调节物质，被发现于 解剖学上与神经纤维非常接近。 这些的数量 经类胰蛋白酶抗体阳性鉴定的细胞增加 青春期后明显遵循相同的发育模式 作为神经纤维。 这些结果证实睾丸 猴子的神经系统由两个部分组成：内在神经 细胞和外在纤维，两者都是儿茶酚胺能的 本质上是肽能的。 此外，这两个组件都高度 塑料在开发过程中，表明可能参与 青春期开始。 神经元样细胞的睾丸内位置 纤维表明儿茶酚胺和神经肽可能具有 多个作用位点，并可能影响 Leydig 细胞、 管壁和血管细胞直接和/或间接通过 肥大细胞的中介作用。 资金 非联邦机构基金 出版物 Lue YH、Sinhahikim AP、Machida Y、Mayerhofer A、Urbanski HF，​​王 C，斯韦德洛夫 RS。 衰老过程中生殖细胞凋亡加速 恒河猴。 内分泌学会第 80 届年度计划和摘要 会议（1998 年 6 月 24-27 日在路易斯安那州新奥尔良举行），第 381 页，1998 年 （抽象的）。 Frunigieri M、Urbanski HF、Calandra R、Mayerhofer A. Elmentos 神经元睾丸 en el mono。 布宜诺斯艾利斯医学中心 58:438，1998（摘要）。