CORE--MOUSE COAGULALTION LABORATORY

核心--小鼠凝血实验室

• 批准号: 6504161
• 负责人: ALVIN H SCHMAIER
• 金额: $ 13.59万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6504161
• 关键词: animal colony; antithrombins; biomedical facility; blood chemistry; blood coagulation; blood donor; clinical research; coagulation factor V; coagulation factor X; fibrinolysis; fibrinopeptide; human subject; immunochemistry; laboratory mouse; phlebotomy; plasminogen activator; prothrombin; von Willebrand factor

The Mouse Coagulation Laboratory will adapt standard clinical coagulation and fibrinolysis assays developed for the analysis of human specimens to the study of mouse samples. Under the direction of Dr. Alvin Schmaier, the director of the University of Michigan Hospitals coagulation Laboratory, this core will provide a centralized resource for the performance by dedicated coagulation laboratory personnel of a series of assays standardized for experimental mice. The specific aims of the Mouse coagulation Laboratory Core are: Specific aim #1: Establish coagulation-based assays with mouse plasma (PT, APTT, thrombin time, fibrinogen, factor assays, APC resistance). Specific aim #2: Establish assays to assess the fibrinolytic system in mouse plasma (whole plasma fibrinolysis plate, tPa and PAI-1 activity and antigen, alpha 2antiplasmin, plasminogen activity and antigen). Specific aim #3: Apply or establish immunochemical assays to measure mouse von Willebrand factor, factor V, factor VIII, fibrinogen, tissue plasminogen activator, plasminogen activator inhibitor-1, thrombin-antithrombin III complexes, and fibrinopeptide A. Establish amidolytic assays for C1 inhibitor and antithrombin III. Specific aim #4: Establish immunochemical assays to measure mouse activation peptides of factor X and prothrombin (Prothrombin fragment 1+2). The services of the Mouse coagulation laboratory will be used by all of the individual projects contained in this PPG proposal. Significant gains in economy, efficiency and accuracy are anticipated to result from consolidation of these activities into one, centralized core laboratory.

小鼠凝血实验室将适应标准临床 开发了用于分析的凝血和纤维蛋白溶解测定 人类的样品研究小鼠样品。 在指导下 密歇根大学主任Alvin Schmaier博士 医院凝血实验室，该核心将提供集中的 专用凝结实验室的绩效资源 一系列针对实验小鼠标准化的测定的人员。 小鼠凝血实验室核心的具体目的是： 特定目的＃1：用小鼠等离子体建立基于凝血的测定 （PT，APTT，凝血酶时间，纤维蛋白原，因子测定，APC 反抗）。 特定目的＃2：建立评估纤维蛋白水解系统的测定 小鼠血浆（整个血浆纤维蛋白溶解板，TPA和PAI-1活性 和抗原，α2抗磷脂，纤溶酶原活性和抗原）。 特定目的＃3：申请或建立免疫化学测定 测量小鼠von willebrand因子，因子V，因子VIII，， 纤维蛋白原，纤溶酶原激活剂，纤溶酶原激活剂 抑制剂1，凝血酶 - 抗凝血酶III复合物和纤维蛋白肽 A.建立C1抑制剂和抗凝血酶III的胺化学测定。 特定目的＃4：建立免疫化学测定以测量鼠标 因子X和凝血酶原的激活肽（凝血酶原片段 1+2）。 鼠标凝血实验室的服务将由所有人使用 在本PPG提案中包含的单个项目中。 重要的 预计经济，效率和准确性会导致 从这些活动的合并到一个集中核心 实验室。