Optical Chip Test For T Cell Function IN HIV Patients

HIV 患者 T 细胞功能的光学芯片测试

• 批准号: 6613712
• 负责人: Bernard H. Schneider
• 金额: $ 38.61万
• 依托单位: NGIMAT COMPANY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6613712
• 关键词: AIDS therapy; HIV infections; bioengineering /biomedical engineering; biological models; biomedical equipment development; biotechnology; cell component structure /function; cell population study; cell sorting; clinical biomedical equipment; cytotoxic T lymphocyte; diagnosis design /evaluation; flow cytometry; functional /structural genomics; gene expression; immunologic assay /test; immunologic memory; laboratory mouse; microarray technology; nonhuman therapy evaluation; optics; polymerase chain reaction; virus RNA; virus replication

DESCRIPTION (provided by applicant): This proposal addresses the need for a simple, low-cost diagnostic to monitor T cell function in HIV-infected patients. Experience with HIV patients undergoing highly active anti-retroviral therapy (HAART) has shown that total T cell count is a useful but insufficient measure of the functional status of the immune system. A more pertinent indicator would be the quantity and quality of HIV-specific memory T cells, and it is believed this subpopulation can be determined by measuring the expression profile of genes linked to memory T cell function. The proposed diagnostic is based on a novel optical chip that is ideally suited for routine, low-density array testing. There is no need for the complex processing steps or expensive analytical instruments associated with conventional high-density array gene chips, and it is expected that the disposable optical chips can be mass-produced for <$5. The Phase I program will use an existing LCMV mouse model to establish feasibility. A prototype optical chip for quantifying the expression profile of several genes linked to CD8 memory T-cell function will be developed and assessed. If successful, a follow-on Phase II program will focus directly on monitoring memory T cell function in HIV-infected humans. PROPOSED COMMERCIAL APPLICATION: A practical, low-cost diagnostic for monitoring HIV-specific T cell function would be a valuable adjunct to T cell counts and viral loads in regulating patients undergoing HAART and other treatment regimens. In addition, the basic test format could easily be applied to other immune-related disorders, pharmacogenomics and clinical trials.

描述（由申请人提供）：该提案解决了对 简单，低成本的诊断，以监测HIV感染的T细胞功能 患者。患有艾滋病毒患者的经验 治疗（HAART）表明总T细胞计数是有用但不足的 衡量免疫系统功能状态。更相关 指标将是HIV特异性记忆T细胞的数量和质量，以及 据认为，可以通过测量表达来确定这种亚群 链接到内存T细胞功能的基因的曲线。建议的诊断是 基于一种新型的光学芯片，非常适合常规，低密度 阵列测试。不需要复杂的处理步骤或昂贵 与常规高密度阵列基因相关的分析仪器 芯片，预计可倾斜的光学芯片可以是 大量生产的价格<$ 5。 I阶段程序将使用现有的LCMV鼠标 建立可行性的模型。原型光芯片，用于量化 链接到CD8存储器T细胞功能的几个基因的表达曲线将 可以开发和评估。如果成功，后续第二阶段计划将 直接专注于监测HIV感染的人类中的记忆T细胞功能。 拟议的商业应用： 用于监测HIV特异性T细胞功能的实用，低成本的诊断将是T细胞计数和病毒载荷的有价值的辅助手段，可调节接受HAART和其他治疗方案的患者。 此外，基本的测试格式可以很容易地应用于其他免疫相关疾病，药物基因组学和临床试验。