RAPID, INEXPENSIVE ASSAY FOR INFLUENZA A

快速、廉价的甲型流感检测

基本信息

批准号：
2069975
负责人：
Bernard H. Schneider
金额：
$ 8.02万
依托单位：
PHOTONIC SENSOR SYSTEMS, INC.
依托单位国家：
美国
项目类别：
财政年份：
1994
资助国家：
美国
起止时间：
1994-06-15 至 1994-12-15
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2069975
关键词：
antigen antibody reaction antiviral antibody avidin biomedical equipment development biosensor device biotin chemical binding immunologic assay /test influenzavirus A interferometry method development monoclonal antibody secretion sputum

项目摘要

This proposal addresses the development of a rapid, inexpensive assay to screen nasopharyngeal secretions or sputum for influenza A. The assay utilizes a novel biosensor, with molecular recognition/binding as the detection element and a proprietary, integrated optic interferometer as the transducer. The unique design of the interferometer minimizes thermomechanicaI noise to provide a highly stable platform for sensing very low concentrations of specific biomolecules, chemicals or microorganisms in either aqueous or gaseous environments. The approach relies on the detection of small refractive index changes at the surface of the interferometer. When receptor molecules are immobilized on the interferometer surface, the subsequent binding of specific ligands results in a pronounced refractive index change. An assay based on this approach has a number of key advantages: l) it is rapid (equal to or less than 15 minutes), 2) it is sensitive (picogram/milliliter range), 3) it is simple (no labeling or tagging) and 4) it is low cost (< $5 unit cost, no expensive analytic instrumentation). The biosensor can be packaged as a hand-held device, suitable for out-of-lab use. Moreover, the technology offers the potential of simultaneously testing for several respiratory infections (e.g.; influenza A, influenza B, RVS, parainfluenza) in a single assay.

该建议涉及开发快速，廉价的测定法鼻咽部分泌物或流感A的痰。利用一种新型生物传感器，具有分子识别/结合为检测元素和专有的，集成的视光干涉仪为传感器。干涉仪的独特设计最小化 ThermoMechaniCai噪声为传感提供了高度稳定的平台非常低浓度的特定生物分子，化学物质或在水性或气态环境中的微生物。方法依赖于表面折射率变化的小折射率变化干涉仪。当受体分子固定在干涉仪表面，随后的特定配体结果的结合在明显的折射率变化中。基于这种方法的测定具有许多关键优势：l）它很快（等于或小于15 分钟），2）它很敏感（皮克图/毫升范围），3）很简单（无标签或标记）和4）价格低廉（<$ 5单位成本，否昂贵的分析仪器）。生物传感器可以包装为手持设备，适用于利用外的设备。而且，技术提供同时测试几种呼吸道的潜力在A 单个测定。