OPTICAL SENSOR FOR MYCOBACTERIUM DETERMINATON

用于分枝杆菌测定的光学传感器

• 批准号: 2887215
• 负责人: Bernard H. Schneider
• 金额: $ 34.56万
• 依托单位: PHOTONIC SENSOR SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-11-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2887215
• 关键词: Mycobacterium; bacterial disease; biomedical equipment development; biosensor device; clinical biomedical equipment; diagnosis design /evaluation; diagnosis quality /standard; early diagnosis; interferometry; nucleic acid hybridization; nucleic acid probes; sputum

This proposal addresses the need for a simple, sensitive screening test for the early detection and differentiation of mycobacterium species in sputum specimens. The proposed diagnostic will combine gene probes specific to mycobacterial ribosomal RNA (16S rRNA) with a proprietary integrated optic interferometer. Hybridization of rRNA to an oligonucleotide probe attached to the interferometer surface results in a refractive index change that can be quantified by the interferometer. Identification of mycobacterium species is achieved by using short gene probes of specific complementary sequences. An optical sensor based on this approach has several key advantages: 1) it allows the simultaneous determination of multiple nucleic acid targets, 2) it is sensitive (potentially <<10(4) cells/mL), 3) it is rapid (60 minute assay), 4) it is simple (no wash steps or subjective interpretation), and 5) it is low cost (<$5 per test, no expensive analytic instrumentation). In particular, the proposed approach should eliminate the need for either time-consuming bacterial culture procedures or complex target amplification methods such as PCR. PROPOSED COMMERCIAL APPLICATIONS: Mycobacterium infections, and tuberculosis in particular, are a major cause of infectious disease-related death on a worldwide scale. The tuberculosis market in the US alone is $75 to $100 million. Conventional methods for identification of the causative organism are slow, and lead to high treatment costs and patient suffering. The proposed optical sensor would enable early diagnosis through increased sensitivity combined with simultaneous multispecies identification.

该建议解决了对简单，敏感的筛选测试的需求 为了早期检测和分化分枝杆菌种类 痰标本。 提出的诊断将结合基因探针 特定于分枝杆菌核糖体RNA（16S rRNA）具有专有的 集成的光学干涉仪。 rRNA杂交 连接到干涉仪表面的寡核苷酸探针导致 可以通过干涉仪量化的折射率变化。 通过使用短基因来实现分枝杆菌物种的鉴定 特定互补序列的探针。基于光学传感器 这种方法具有几个关键优势：1）允许同时 确定多种核酸靶标，2）它是敏感的 （潜在的<< 10（4）个单元/ml），3）它是快速（60分钟测定），4） 很简单（没有洗涤步骤或主观解释），5）它很低 成本（每次测试<$ 5，没有昂贵的分析仪器）。在 特别，提议的方法应消除对任何一种的需求 耗时的细菌培养程序或复杂靶标 放大方法，例如PCR。 拟议的商业应用： 分枝杆菌感染，尤其是结核病是主要的 在全球范围内导致与传染病有关的死亡原因。这 仅美国的结核病市场就是75至1亿美元。传统的 鉴定因果生物的方法很慢，并且领导 高治疗费用和患者痛苦。 提出的光学 传感器将通过提高灵敏度实现早期诊断 结合同时的多人识别。