MECHANISMS OF IMPLANTATION OF BLASTOCYST

囊胚植入机制

• 批准号: 6116646
• 负责人: ALLEN C ENDERS
• 金额: $ 5.91万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6116646
• 关键词: animal tissue; growth /development; reproductive system

Significance Implantation of the blastocyst in the maternal endometrium follows a specific sequence in different species of mammals. Nonhuman primates show similarities between species and with the human with regard to syncytial trophoblast formation, formation of the trophoblastic plate, and lacunar and villus formation. We have determined that the cytotrophoblast involved in formation of the trophoblastic shell demonstrates surface features which appear to enable this trophoblast to invade maternal arteries as well as endometrial stroma. Objectives The objective of this project is to understand more completely the processes involved in implantation of the blastocyst in the endometrium in non-human primates. Studies focus on cellular interactions of the trophoblast and endometrium, and on differentiation of the early post implantation embryo and trophoblast. Results Macaque implantation sites were examined by electron microscopy and immuno-cytochemistry, focussing on modification of the wall of maternal arteries by endovascular cytotrophoblast. Immunostaining of cell adhesion molecules combined with localization of constituents that indicate cell type showed a sequence of changes in the vessel wall as cytotrophoblast migrated and invaded. Electron microscopic examination of the vessels showed that intramural cytotrophoblast secretes a matrix which excludes maternal elements, resulting in an intramural pad of cytotrophoblast. The studies also described the way in which the arteries, especially the muscle layers of the vessels, become remodeled. Future Directions Although cytotrophoblast modifies maternal arteries, the first invasion of the uterine epithelium is by syncytial trophoblast. It is not yet known what cell surface factors facilitate invasion of this epithelium and of maternal vessels. We will attempt to obtain some pre-lacunar implantation stages in order to assess the invasive characteristics of trophoblast types in the first days of implantation. KEYWORDS placenta, trophoblast, endometrium, immunocytochemistry FUNDING NIH Grant HD10342

囊胚植入母体的意义 不同物种的子宫内膜遵循特定的序列 哺乳动物。 非人类灵长类动物表现出物种之间的相似性 人类关于合体滋养层形成、 滋养层板、腔隙和绒毛形成。 我们有 确定细胞滋养层参与形成 滋养层外壳表现出的表面特征似乎 使滋养层能够侵入母体动脉以及 子宫内膜间质。 目标 该项目的目标是 更全面地了解植入所涉及的过程 非人类灵长类动物子宫内膜中的囊胚。 研究 重点关注滋养层和子宫内膜的细胞相互作用，以及 着床后早期胚胎的分化 滋养层。 结果通过以下方法检查了猕猴植入部位： 电子显微镜和免疫细胞化学，重点关注 血管内修复母体动脉壁 细胞滋养层。 结合细胞粘附分子的免疫染色 指示细胞类型的成分的定位显示 随着细胞滋养层迁移和迁移，血管壁发生一系列变化 入侵了。 对血管的电子显微镜检查表明 壁内细胞滋养层分泌一种基质，排除母体 元素，形成细胞滋养层的壁内垫。 这 研究还描述了动脉，尤其是动脉的方式 血管的肌肉层被重塑。 未来的方向 尽管细胞滋养层改变母体动脉， 子宫上皮的侵入是由合体滋养层进行的。 这是 尚不知道哪些细胞表面因素促进了这种入侵 上皮和母体血管。 我们将尝试获得一些 腔隙前植入阶段，以评估侵入性 出生后第一天滋养层类型的特征 植入。 关键词 胎盘，滋养层，子宫内膜， 免疫细胞化学资助 NIH 格兰特 HD10342