MECHANISMS OF IMPLANTATION OF THE BLASTOCYST

囊胚植入机制

• 批准号: 3311278
• 负责人: ALLEN C ENDERS
• 金额: $ 21.93万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-04-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3311278
• 关键词: Cercopithecidae; Macaca mulatta; autoradiography; biological models; cell adhesion; cell cell interaction; cell fusion; cytology; early embryonic stage; electron microscopy; embryo implantation; endometrium; fluorescent dye /probe; guinea pigs; histochemistry /cytochemistry; intermediate filaments; laboratory mouse; laboratory rat; mammalian embryology; membrane activity; radiotracer; receptor mediated endocytosis; scanning electron microscopy; trophoblast; urinalysis; uterus

The long range objective of this project is to understand the processes involved in interaction of the trophoblast and endometrium during implantation. Advances in our understanding of the endocrine control of pregnancy and of in vitro fertilization in the human have not been paralleled by increased understanding of implantation, due in part to a lack of information concerning early implantation and of suitable in vivo and in vitro models. Our first aim is based on our hypothesis that different types of trophoblast play different roles in implantation, and that consequently the extent and duration of a given type of trophoblast, and endometrial responses to these types, determine the nature of the implantation process. To examine the characteristics of trophoblast participating in implantation in nonhuman primates, immunocytochemical methods will be used to localize intermediate filament types in early implantation stages obtained from cynomolgus or rhesus monkeys. Distribution of antibody to chorionic gonadotropin and to factor VIII-related antigen, and the distribution of lysosomal constituents will also be used to determine differences in origin and function of the different trophoblast types. In vitro incorporation of 3H thymidine and 3H leucine will be used to determine the potential for cell replication and growth. Several methods will be used to examine in vitro interactions with specific matrices. The studies of trophoblast types are expected to correlate with numerous studies concerning trophoblst cells derived from first trimester human placentas. They should clarify what aspects of the implantation process in nonhuman primates are similar, different, or exaggerated, compared with the human. To characterize the first adhesion between trophoblast and uterine luminal epithelium, initial adhesion and epithelial penetration stages will be obtained from cynomolgus monkeys. To model interactions between trophoblast and uterine epithelium, rat trophoblast cells will be exposed in vitro to different polarized epithelia. To examine the role of decidual cells in implantation, decidual cell interactions with extracellular matrix will be examined in vitro, and the nature of the interaction with basal laminas will be explored using immunocytochemical methods. Structural methods will be used to examine the manner in which trophoblast penetrates capillaries in the primate, and the manner in which pathways for maternal blood are established in the rat. To continue studies of differentiation prior to implantation, and attempt will be made to follow the fate of individual blastomeres using differential interference contrast or vital fluorescent dyes. Studies concerning formation of the primate embryo will focus on the origin of intraembryonic endoderm and mesoderm.

该项目的长期目标是了解 涉及滋养层和滋养层相互作用的过程 着床期间的子宫内膜。 我们理解的进步 妊娠和体外受精的内分泌控制 人类的理解力还没有得到相应的提高 植入的部分原因是缺乏相关信息 早期植入以及合适的体内和体外模型。 我们的首要目标是基于我们的假设，即不同类型的 滋养层在着床过程中发挥着不同的作用 因此，特定类型的程度和持续时间 滋养层和子宫内膜对这些类型的反应决定 植入过程的性质。 要检查 滋养层参与着床的特点 非人类灵长类动物，免疫细胞化学方法将用于 在早期植入阶段定位中间丝类型 从食蟹猴或恒河猴获得。 分布 绒毛膜促性腺激素和因子 VIII 相关抗体 抗原，溶酶体成分的分布也将 用于确定起源和功能的差异 不同的滋养层类型。 3H 的体外掺入 胸苷和 3H 亮氨酸将用于确定潜力 用于细胞复制和生长。 将使用多种方法 检查与特定基质的体外相互作用。 研究 滋养层类型预计与许多 有关源自妊娠早期的滋养层细胞的研究 人类胎盘。 他们应该澄清哪些方面 非人类灵长类动物的着床过程有相似之处，也有不同之处， 与人类相比，或者说是夸张的。 表征滋养层和滋养层之间的首次粘附 子宫腔上皮、初始粘连和上皮 渗透阶段将从食蟹猴获得。 到 滋养层和子宫上皮之间的相互作用模型， 大鼠滋养层细胞将在体外暴露于不同的极化 上皮细胞。 为了检查蜕膜细胞在着床中的作用， 蜕膜细胞与细胞外基质的相互作用 体外检查，以及与基础相互作用的性质 将使用免疫细胞化学方法探索层板。 将使用结构方法来检查 灵长类动物的滋养层穿透毛细血管的方式 其中在大鼠体内建立了母血通路。 继续研究植入前的分化，以及 将尝试追踪单个卵裂球的命运 使用微分干涉对比或活性荧光染料。 关于灵长类动物胚胎形成的研究将集中于 胚胎内内胚层和中胚层的起源。